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Publication
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| Title of Article |
Co-localization of endogenous Arf6 and its activator EFA6D in the granular
convoluted tubule cells of mouse submandibular glands under normal
conditions and when stimulated by isoproterenol, noradrenaline and
carbachol |
| Date of Acceptance |
10 June 2017 |
| Journal |
| Title of Journal |
Archives of Oral Biology |
| Standard |
SCOPUS |
| Institute of Journal |
ELSEVIER |
| ISBN/ISSN |
0003-9969 |
| Volume |
- |
| Issue |
82 |
| Month |
october |
| Year of Publication |
2017 |
| Page |
153-159 |
| Abstract |
Objective: This study proposed to investigate the localization at light and electron microscopic levels of Arf6 and
its activator EFA6D in the mouse submandibular gland (SMG) under normal conditions and when stimulated by
adrenergic or cholinergic agonists.
Materials and methods: SMGs of male adult mice were utilized for immunoblotting and immuno-light and
-electron microscopic analyses. Isoproterenol and noradrenalin were used as adrenergics, while carbachol was
used for the cholinergic stimulant. SMGs were examined at 15, 30, 60 and 120 min after intraperitoneal injection
of these agents.
Results: Immunoreactivities for both Arf6 and its activator EFA6D were similarly intense in the basolateral
domain of GCTs, but no significant immunoreactivities were seen in the apical domain of GCT cells or any
domain of acinar cells under normal conditions. In immuno-electron microscopy, the immunoreactive materials
were mainly deposited on the basolateral plasma membranes and subjacent cytoplasm. Shortly after injection of
isoproterenol and noradrenaline, but not carbachol, the immunoreactivities for both molecules were additionally
seen on the apical plasmalemma of most, if not all, GCT cells, but not acinar cells.
Conclusion: The present findings suggest that the direct involvement of Arf6/EFA6D in regulatory exocytosis at
the apical plasma membrane of acinar and GCT cells is apparently to be smaller, if present, than that of endocytosis
at the basolateral membranes of GCT cells under normal conditions. This also suggests that the two
molecules function additionally at the apical membrane of GCT cells for modulation of saliva secretion under β-
adrenoceptor stimulation. |
| Keyword |
Arf6 EFA6D Submandibular gland Granular convoluted tubule Basolateral cell domain |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Attach file |
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| Citation |
0
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Journal Publication
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