|
|
Publication
|
| Research Title |
Antimicrobial peptide analogs of Crocodylus siamensis Leucrocin I revealing anticancer activity and apoptotic induction on human colon cancer HCT-116 cells |
| Date of Distribution |
11 July 2017 |
| Conference |
| Title of the Conference |
The 5th Asia Pacific Protein Association (APPA) Conference and the 12th International Symposium for the Protein Society of Thailand (PST) |
| Organiser |
The Protein Society of Thailand (PST), the Chulabhorn Research Institute (CRI) and Burapha University. |
| Conference Place |
The Tide Resort |
| Province/State |
Bangsaen, Thailand |
| Conference Date |
11 July 2017 |
| To |
14 July 2017 |
| Proceeding Paper |
| Volume |
2017 |
| Issue |
12 |
| Page |
146 |
| Editors/edition/publisher |
|
| Abstract |
Colorectal cancer constitutes the third most common type of cancer worldwide. Conventional cancer treatment strategies involving chemotherapeutic drugs have been associated with pronounced detrimental side effects against normal cells and tissues and may frequently display low efficacy due to increasing drug resistance of specific cancer cell lines. Consequently, these problems lead to a decrease in the potential and add to the unsatisfactory nature of anticancer therapeutics, thus emphasizing the urgent need for the discovery of new drugs for cancer therapy. In light of this evidence, the present study aimed to introduce several specific cationic Leucrocin I analogs with antimicrobial properties (i.e. NY7, NY15, KT2 and RT2), which are non-toxic to normal mammalian cells while exhibiting cytotoxic activity against cancer cells. Firstly, the anticancer activity of a set of known potent antimicrobial Leucrocin I analogs against the HCT-116 colon cancer cell line was investigated employing an MTT assay with non-cancerous Vero cells as the control. The results indicated that among the 4 investigated peptides only the KT2 and RT2 analogs exhibited anticancer activity. The IC50 values of KT2 and RT2 were determined as 79.67 and 99.25 µg/ml, respectively. Moreover, an in-vitro scratch migration assay revealed that peptides KT2 and RT2 were able to suppress cancer cell migration to a remarkable extent, indicating potent anti-metastatic properties. The mechanism involved in cancer cell cytotoxicity of both peptides was shown to be associated with the induction of apoptosis, as evidenced by acridine orange/ethidium bromide (AO/EB) fluorescent staining and annexin V-FITC/PI staining flow cytometry analysis. From the collected results it is concluded that the antimicrobially active peptides KT2 and RT2 also possess significant potency in killing human colon cancer cells. |
| Author |
|
| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย