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Publication
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| Title of Article |
Impact of high fat- and high fructose-diet on murine hepatic histological feature and CYP3A11 profile |
| Date of Acceptance |
28 March 2017 |
| Journal |
| Title of Journal |
Isan Journal of Pharmaceutical Sciences, IJPS |
| Standard |
TCI |
| Institute of Journal |
Faculty of Pharmaceutical Sciences, Khon Kaen University |
| ISBN/ISSN |
ISSN : 1905-0852 |
| Volume |
13 |
| Issue |
1 |
| Month |
January- March |
| Year of Publication |
2017 |
| Page |
71-80 |
| Abstract |
Fructose and hydrogenated oil appeared as ingredients in several processed food are risk factors to develop metabolic diseases. Liver is a major organ which is impacted by these diets. Cytochrome P450 3A (CYP3A) is a major hepatic cytochrome P450 enzyme that takes responsible for metabolism of almost clinical drugs. The present study aimed to investigate hepatic histology and CYP3A11 pattern in mice fed with high fat- and high fructose-diet (HFFD). Methods: Seven-week-old male ICR mice (n=5) were intragastrically administered hydrogenated soybean oil (1 mL/day) with free access to 20% (w/v) aqueous fructose solution for 2, 4, and 8 weeks. The protein expression and catalytic activity of CYP3A11 were determined employing immunoblotting techniques and a specific reaction of erythromycin N-demethylation (ENDM), respectively. Results: Karyorrhexis and pyknosis of nuclei were detected after the HFFD feeding for 4 weeks while Kupffer cells in sinusoid and microvesicular hepatocytes were observed after 8 weeks of the induction. The change of hepatic histological features was correlated with an increase in the protein level and catalytic activity of CYP3A11. Moreover, the fasting blood glucose level and AUC in the oral glucose tolerance test were increased in the HFFD-fed mice at the end of the study. Conclusion: High fat- and high fructose-consumption worsen the physiological feature of hepatic tissue along with induction of murine CYP3A11 profile, though it did not affect the weight gaining. Hence, a person with long-term consumption of high fat- and/or high fructose-diet could have a risk of hepatic pathology and induction of CYP3A11 expression, leading to alter drug metabolism and extensive risk of drug interaction. |
| Keyword |
CYP3A11, Histology, High fat diet, High fructose diet |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
ชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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