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Publication
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| Research Title |
Moringa oleifera leaf extract alleviates oxidative stress in rats with L-NAME-induced hypertension |
| Date of Distribution |
5 August 2017 |
| Conference |
| Title of the Conference |
The 1st international conference on natural medicine: "From Local Wisdom to International Research" |
| Organiser |
Ubon Ratchathani University, Khon Kaen University, Naresuan University, and Institute of Natural Medicine, University of Toyama, Japan |
| Conference Place |
The Sukosol Hotel |
| Province/State |
Bankok, Thailand |
| Conference Date |
5 August 2017 |
| To |
6 August 2017 |
| Proceeding Paper |
| Volume |
2017 |
| Issue |
1 |
| Page |
28 |
| Editors/edition/publisher |
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| Abstract |
Reactive oxygen species (ROS)-induced oxidative stress has been suggested to play a role in pathogenesis of hypertension. There is considerable evidence that dietary antioxidants can protect against cardiovascular disease. The leaf of Moringa oleifera (local Thai name: Marum) is used as decoctions in traditional Thai medicine for treatment cardiovascular problems. This study investigated whether aqueous extract of M. oleifera leaves (MOE) could prevent Nω-nitro-L-arginine-methyl ester (L-NAME)-induced high blood pressure and oxidative stress in rats. In in vitro experiment using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, MOE showed a free radical scavenging activity, however, with potency 5 times less than ascorbic acid. In ferric-reducing antioxidant power (FRAP) assay, the extract had relatively strong reductant capacity with FRAP value of 422.8 ± 6.9 μg ascorbic acid/mg extract. In in vivo experiments, male Wistar rats were administered with 50 mg/kg/day of L-NAME in drinking water for three weeks to induce hypertension. At the end of experiment, systolic blood pressure (SBP) was measured in anesthetized rats via femoral artery catheterization. L-NAME treated rats developed significant increases in SBP compared to normal control rats (193.6 ± 1.6 vs. 122.9 ± 1.4 mmHg, P<0.05). Hypertensive rats had increased oxidative stress (P<0.05) as indicated by increased vascular superoxide production (130.3 ± 1.4 vs. 44.6 ± 2.1 counts/mg dry weight/min) and plasma malondialdehyde (MDA) levels (10.2 ± 0.4 vs. 4.2 ± 0.2 µM). Concurrent oral treatment with MOE at 30 and 60 mg/kg/day displayed dose-dependent decreases in SBP by 175.7 ± 1.9 and 149.0 ± 1.8, respectively (P<0.05). Interestingly, the antihypertensive effect of MOE treatment was associated with suppression of superoxide production and reduction of MDA levels (P<0.05). These findings suggest that MOE may decrease the high blood pressure via its antioxidant activity in L-NAME-induced hypertensive rats. Thus, MOE may be useful as a dietary supplement against hypertension and oxidative stress. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย