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Publication
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| Title of Article |
A simple solvent based microextraction for high performance liquid
chromatographic analysis of aflatoxins in rice samples |
| Date of Acceptance |
7 August 2017 |
| Journal |
| Title of Journal |
Talanta |
| Standard |
ISI |
| Institute of Journal |
elsevier |
| ISBN/ISSN |
ISSN: 0039-9140 |
| Volume |
2018 |
| Issue |
176 |
| Month |
January |
| Year of Publication |
2017 |
| Page |
172-177 |
| Abstract |
This paper describes the development of a simple solvent based microextraction, namely vortex assisted low
density solvent–microextraction (VALDS–ME), followed by high performance liquid chromatography-fluorescence
detection (HPLC–FD) for the simultaneous determination of four aflatoxins (AFs) including AFB1, AFB2,
AFG1 and AFG2 in rice samples. In VALDS–ME, a mixture of low density solvents (1–octanol and toluene) was
used as the extraction solvent. The extraction was rapidly achieved with the assistance of vortex agitation and
phase separation was easily obtained after the addition of Na2SO4. The effects of various parameters on the
extraction efficiency were optimized. Under the optimum conditions, high enrichment factors (42–132), low
limits of detection (LODs) in the range of 0.0011–0.17 μg kg−1 and good precisions (RSDs lower than 6.2%)
were obtained. AFB1 and AFG1 were detected in berry rice sample at 0.26 and 2.1 μg kg−1, respectively. The
recoveries in AFs-spiked rice samples ranged from 70% to 104%. Moreover, the present method was
comparable to the conventional immunoaffinity chromatography method. |
| Keyword |
Aflatoxins, Vortex assisted low density solvent microextraction, High performance liquid chromatography, Fluorescence detection, Rice |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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