| Research Title |
Induce mutation of thermotolerant yeast Saccharomyces cerevisiae DBKKUY-53 using ethyl methane sulfonate |
| Date of Distribution |
25 July 2017 |
| Conference |
| Title of the Conference |
The 7th International Conference on Fermentation Technology for Value Added Agricultural Product |
| Organiser |
Fermentation Research Center For Value Added Agricultural Products |
| Conference Place |
Pullman Khonkaen Raja Orchid |
| Province/State |
Khonhaen |
| Conference Date |
25 July 2017 |
| To |
28 July 2017 |
| Proceeding Paper |
| Volume |
7 |
| Issue |
1 |
| Page |
116-119 |
| Editors/edition/publisher |
|
| Abstract |
Mutation of the thermotolerant yeast
Saccharomyces cerevisiae DBKKUY-53 was
induced using ethyl methane sulfonate (EMS)
and its mutant strains that capable of growth at
high sugar concentrations were screened using
yeast extract malt extract (YM) medium
containing different glucose concentrations. S.
cerevisiae wild-type strain (DBKKUY-53)
exhibited a good growth in the YM medium
containing 250 and 300 g/L glucose. When
sugar concentration in the YM medium
increased to 350 and 400 g/L, growth of the
yeast strain was reduced. By using EMS
mutagenization, 42 isolates of the S. cerevisiae
mutant strains were obtained. Comparative
study on growth performance at high
temperature (37C) of the selected mutant and
wild-type strains using YM medium
containing 350 g/L glucose was performed. As
found in the current study, a mutant strain
designated S. cerevisiae EMS33 exhibited a
good growth under a selective condition.
Morphological analysis and growth properties
of the selected mutant strains were performed
and described in this work.
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| Author |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
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