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Journal Publication
Research Title Moringa oleifera leaf extract causes endothelium-dependent vasodilatation through NO-sGC and H2S-KATP pathways 
Date of Distribution 6 December 2017 
     Title of the Conference 45th PHYSIOLOGICAL SOCIETY OF THAILAND ANNUAL MEETING: "Translational Physiology: Implications in Health and Disease" 
     Organiser Department of Physiology, Faculty of Medicine at Khon Kaen University and the Physiological Society of Thailand 
     Conference Place The AVANI Khon Kaen Hotel and Convention Centre Khon Kaen, Thailand 
     Province/State Khon Kaen 
     Conference Date 6 December 2017 
     To 8 December 2017 
Proceeding Paper
     Volume 45 
     Page 38 
     Abstract Hypotensive effect of aqueous extract of Moringa oleifera leaf extract (MOE) has been reported, although little is known regarding its effect on resistance arterioles. The objectives of this study was to investigate the vasorelaxant activity of MOE on resistance arteries isolated from male Wistar rats, and to gain insight into its mechanism(s) focusing on endothelium-dependent vasodilatory action. In vitro experiments on perfused mesenteric arterial beds precontracted by methoxamine revealed that injection of MOE (0.001-0.3 mg) into the perfusate caused a concentration-dependent vasorelaxation which was abolished by endothelium denudation. The endothelium-dependent vasorelaxation of MOE was partially, but significantly inhibited by nonselective nitric oxide (NO) synthase inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), as well as the soluble guanylate cyclase (sGC) inhibitor 1H-1,2,3]oxadiazolo[4,4-a] quinoxalin-1-one (ODQ). Moreover, inhibition of the hydrogen sulfide (H2S) producing enzyme cystathionine-gamma-lyase (CSE) by DL-propargylglycine (PAG) significantly abolished MOE endothelium-dependent response. On the other hand, vasodilatory effect was not significantly affected by the cyclooxygenase (COX) inhibitor indomethacin (INDO). In presence of coapplication of L-NAME and INDO, the persisting MOE endothelium-dependent relaxation was completely inhibited by the ATP-dependent potassium channel (KATP) blocker glibenclamide comparable to MOE response in denuded preparations. This study provided the first evidence of in vitro vasorelaxant action of MOE indicating that the relaxant activity of MOE on rat mesenteric artery predominantly relies on endothelium-dependent signaling likely through NOsGC and H2S-KATP pathways. Additional mechanisms underlying endothelium-independent vasorelaxation of MOE await further investigations. MOE has the potential for developing as a medicinal natural substance in the treatment of cardiovascular diseases through its vasodilatory and antihypertensive action. 
577070012-2 Mr. DIREK AEKTHAMMARAT [Main Author]
Medicine Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference ชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis true 
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