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Publication
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| Title of Article |
Aberrant gene promoter methylation of E-cadherin, p16INK4a, p14ARF, and MGMT in Epstein–Barr virus-associated oral squamous cell carcinomas |
| Date of Acceptance |
24 May 2017 |
| Journal |
| Title of Journal |
Medical Oncology |
| Standard |
ISI |
| Institute of Journal |
Springer Science+Business Media New York 2017 |
| ISBN/ISSN |
1357-0560 |
| Volume |
34 |
| Issue |
7 |
| Month |
July |
| Year of Publication |
2017 |
| Page |
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| Abstract |
The etiology of oral carcinogenesis appears to be multifactorial. There is emerging evidence of the presence of Epstein–Barr virus (EBV) in epithelial oral squamous cell carcinoma (OSCC), but an association of EBV with oral carcinogenesis has not yet been established. Although epigenetic alterations, such as aberrant DNA methylation,are known to contribute to the pathogenesis of oral cancer, the relationship of such alterations with EBV infection is little known. This study aimed to investigate the association between EBV infection and promoter methylation patterns of tumor-associated genes in OSCC tissues. A total of 165 of formalin-fixed paraffin-embedded OSCC tissues were studied (68 of EBV positive and 97 of EBV negative). The promoter methylation patterns were investigated for four tumor-associated genes, E-cadherin, p16INK4a, p14ARF, and MGMT, by using methylation-specific polymerase chain reaction (MSP). The frequencies of gene promoter hypermethylation in all cases were 47.3% for Ecadherin, 92.7% for p16INK4a, 74.5% for p14ARF, and 35.8% for MGMT. Interestingly, most of the analyzed gene promoters were more frequently hypermethylated in EBVpositive than EBV-negative cases, in particular the Ecadherin (56/22) and MGMT (38/21) gene promoters (p < 0.05). Concomitantly, hypermethylation of multiple gene promoters (>3) was encountered more frequently in EBV-positive samples. Hypermethylation of the E-cadherin promoter associated with EBV was more frequently observed in moderately and poorly differentiated OSCC tissues. These results indicate that epigenetic changes frequently occur in OSCCs and may partly be induced by EBV infection, therefore, EBV may involve in development and progression of the OSCCs. |
| Keyword |
Epstein–Barr virus, Promoter methylation, Oral squamous cell carcinoma, Epigenetic changes |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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