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Publication
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| Research Title |
Induction of Follicular Growth and Atresia: Expression of Aromatase mRNA in the Ovary of Bos Indicus |
| Date of Distribution |
1 November 2017 |
| Conference |
| Title of the Conference |
The 2nd International Conference on Animal Nutrition and Environment (ANI-NUE2017) |
| Organiser |
Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University |
| Conference Place |
Pullman Raja Orchid Hotel, Khon Kaen, Thailand |
| Province/State |
Khon Kaen, Thailand |
| Conference Date |
1 November 2017 |
| To |
4 November 2017 |
| Proceeding Paper |
| Volume |
2 |
| Issue |
1 |
| Page |
307-311 |
| Editors/edition/publisher |
Cherdthong A., Foiklang S., Mapat, C., Pilajun R., Kang SC. and Wanapat M. |
| Abstract |
Aromatase activity and follicular estradiol (E2) concentration are significantly increased during the initiation of the bovine preovulatory LH surge and correlated with the developmental stages of follicular growth. However, in follicular atresia, the expression of aromatase mRNA is not well reported especially in bovine. The objective of this study was to determine effect of FSH or FSH-saline treatments on follicular growth and atresia, expression of aromatase mRNA, and peripheral E2 concentrations in beef cows. Twelve mature Brahman crossbred cows, (24-30 months of age; 250-300 kg of body weight) were randomly to received intramuscular FSH injections (Ovagen™, ICPbio Reproduction, Auckland, New Zealand) for 3 days to induce follicular growth (decreasing daily doses of 10, 8, 6 mg on days 17, 18 and 19) or FSH-saline to induce follicular atresia (10 mg FSH on day 17 and normal saline on days 18 and 19) or saline injections for 3 days as a control. Treatment of 3 days FSH increased (P<0.05) total numbers of follicles per cow compared to the others (21.3 ± 0.4, 7.5 ± 0.3, and 4.0 ± 0.4 for 3 days FSH, 1 day FSH, and saline, respectively). Expression of aromatase mRNA in granulosa cells was greatest (P<0.05) in cows received 3 days FSH compared to cows received 1 day FSH and the control cows. Concentrations of E2, in pooled follicular fluids samples in small and medium follicles, were also greatest (P<0.05) in cows received FSH for 3 days, but were not different (P>0.05) in large follicles. This study indicates that induction of bovine follicular growth and atresia can be conducted using FSH and FSH-saline treatments. The expression of aromatase mRNA, follicular E2 concentrations are involved in the final growth or atresia of preovulatory follicle. This FSH induced model will provide the useful for studying the mechanisms regulating bovine follicular growth. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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