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Publication
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| Research Title |
Improvement of butanol fermentation using co-cultures |
| Date of Distribution |
18 November 2017 |
| Conference |
| Title of the Conference |
13th Young Scientist Seminar: Establishment of International Network for Tropical Bioresources and Their Utilization |
| Organiser |
Yamaguchi University |
| Conference Place |
Yamaguchi Prefectural Seminar Park, Yamaguchi, Japan |
| Province/State |
Yamaguchi |
| Conference Date |
18 November 2017 |
| To |
19 November 2017 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
32 |
| Editors/edition/publisher |
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| Abstract |
Butanol, a 4-carbon alcohol, is an excellent biofuel compared to the currently popular fuel additive, ethanol. It has several advantages over ethanol, such as its higher miscibility with gasoline and diesel fuel. Butanol can be produced by fermentation process called acetone–butanol–ethanol (ABE) fermentation of Clostridium spp. under strictly anaerobic condition. To reduce the cost of butanol production, the butanol fermentation by co-cultures of Clostridium beijerinckii TISTR1461 and Arthrobacter sp. was studied, and the butanol fermentation in a stirred tank fermenter and a low-cost bioreactor (a bubble column bioreactor) were compared. In co-culture conditions, Arthrobacter sp. was used to create strictly anaerobic condition, whereas C. beijerinckii TISTR1461 was used as a butanol producer. Sugarcane molasses containing 50 g L-1 of total sugar was supplemented with dried spent yeast at 6 g L-1, and was used as substrate for ABE fermentation. In control experiment, nitrogen flushing was used to create anaerobic condition, and single culture of C. beijerinckii TISTR1461 was used to produce butanol. The fermentation was carried out in a 1-L screw capped bottle. The results showed that butanol concentration (PB) and butanol productivity (QB) of the control experiment were 8.67 g L-1 and 0.24 g L-1 h-1, respectively. Under co-culture conditions, Arthrobacter sp. was first inoculated into the molasses medium, then C. beijerinckii TISTR1461 was transferred into the medium at varying times (0-24 h of fermentation). The results showed that the simultaneous addition of C. beijerinckii TISTR1461 and Arthrobacter sp. gave the highest PB (8.81 g L-1) and QB (0.24 g L-1 h-1). These values were not different to those of the control experiment, indicating that Arthrobacter sp. could be used to create anaerobic condition instead of using nitrogen flushing method. When the butanol production by the co-cultures was performed in a 2-L stirred-tank fermenter, the PB and QB were slightly increased to 9.95 g L-1 and 0.29 g L-1 h-1, respectively. This might be due to better mixing in the fermenter. When the butanol production by the co-cultures was performed in a 1-L bubble column bioreactor, the PB and QB were slightly lower to 8.50 g L-1 and 0.24 g L-1 h-1, respectively. The results indicated that the low-cost bioreactor or bubble column bioreactor could be used to produce butanol instead of using the more expensive stirred-tank fermenter. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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