| Research Title |
Moringa Oleifera Leaf Extract Promotes Endothelium-Dependent Vasorelaxation by Inhibiting Oxidative Stress and Stimulating the Releases of NO and H2S |
| Date of Distribution |
27 April 2018 |
| Conference |
| Title of the Conference |
การประชุมวิชาการประจำปีสมาคมเภสัชวิทยาแห่งประเทศไทยครั้งที่ 40 “นวัตกรรมทางเภสัชวิทยา (Innovation in Pharmacology)” |
| Organiser |
ภาควิชาเภสัชวิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยมหิดล ร่วมกับสมาคมเภสัชวิทยาแห่งประเทศไทย |
| Conference Place |
ห้องบรรยายรวม L01 ตึกกลม คณะวิทยาศาสตร์ มหาวิทยาลัยมหิดล พญาไท |
| Province/State |
กรุงเทพ |
| Conference Date |
26 April 2018 |
| To |
28 April 2018 |
| Proceeding Paper |
| Volume |
40 |
| Issue |
1 |
| Page |
11 |
| Editors/edition/publisher |
นพวรรณ ภู่มาลา มอเรส และพรพรรณ วิวิธนาภรณ์ |
| Abstract |
Beneficial effects of Moringa oleifera leaf extract have been reported in cardiovascular diseases but little is known regarding its pharmacological effect on vascular function. This study investigated the antihypertensive effect of aqueous extract of M. oleifera leaves (MOE) in hypertensive rats and explored its endothelium-dependent vasorelaxant action. Male Wistar rats were given the nitric oxide (NO) synthase inhibitor Nω-nitro-L-arginine-methyl ester (L-NAME, 50 mg/kg/day) in drinking water for 3 weeks to induce hypertension. Systolic blood pressure (SBP) was measured in conscious rats by tail-cuff plethysmography. Vasorelaxant reactivity was determined by measuring the reduction in perfusion pressure in isolated mesenteric arterial beds. Vascular oxidative stress biomarkers including superoxide anion (O2•) production and malondialdehyde (MDA) levels were determined by lucigenin-enhanced chemiluminescence method and thiobarbituric acid-reactive substance assay, respectively. The results showed that L-NAME administered rats had high SBP, and increased vascular O2• and MDA production. In vascular beds of the L-NAME administered rats, acetylcholine-induced endothelium-dependent relaxation was impaired. Interestingly, these abnormalities: the high blood pressure, increased oxidative stress and endothelium dysfunction, were attenuated by concurrent oral MOE (30 and 60 mg/kg/day). Moreover, MOE (0.001-3 mg in 0.1 ml injection volume) caused concentration-dependent relaxation in methoxamine-induced vasoconstriction with a maximum relaxation (Rmax) of 95±1% achieved at 3 mg. The vasorelaxation produced by MOE was abolished by endothelium denudation (Rmax = 43±4%) or pretreatment with either NO-synthesis inhibitor L-NAME (Rmax = 72±4%), or hydrogen sulfide (H2S)-synthesis inhibitor DL-propargylglycine (Rmax = 83±2%). But, the MOE-induced relaxation was not affected by the cyclooxygenase inhibitor, indomethacin (Rmax = 93±1%). These results suggest that the antihypertensive effect of MOE may be mediated by inhibition of oxidative stress-induced endothelium dysfunction, and stimulation of endothelium-dependent relaxation of resistance arteries. The endothelium-dependent vasorelaxation in response to MOE is likely mediated by NO and H2S. MOE has a potential to be developed as a natural product against hypertension. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
ผู้ได้รับคัดเลือกให้นำเสนอผลงานด้วยวาจา |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
สมาคมเภสัชวิทยาแห่งประเทศไทย |
| Date of awarding |
27 เมษายน 2561 |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย