| TH, publication_article_article_name |
Antioxidant Capacity of Momordica charantia Extract
and its Protective Effect on Testicular Damage in
Valproic Acid-Induced Rats |
| TH, publication_article_accepted_date |
30 January 2018 |
| TH, publication_article_journal |
| TH, publication_article_journal_name |
International Journal of Morphology |
| TH, publication_article_journal_standard |
ISI |
| TH, publication_article_institute |
International Journal of Morphology, Faculty of Medicine, Universidad de La Frontera, Casilla 54-D, Temuco, Chile. |
| TH, publication_article_isbn |
|
| TH, publication_article_year |
36 |
| TH, publication_article_issue |
2 |
| TH, publication_article_month |
May |
| TH, publication_article_print_year |
2018 |
| TH, publication_article_page |
447-453 |
| TH, publication_article_abstract |
SUMMARY: Although Momordica charantia (MC) has preventive effects on tissue injuries, antioxidant capacity and protective
effect of MC pulp and peel (MCP) on valproic acid (VPA)-testicular damage have never been reported. Fresh MCPs were aqueous
extracted and determined for antioxidant capacity and momordicine I level by HPLC. Male rats were divided into 5 groups (control, VPA
(500 mg/kgBW), MCP20/40/ or 80 mg/kgBW+VPA). In 30 experimental days, animals were pretreated with different doses of MCPs for
20 days before VPA injection for 10 consecutive days. Sperm concentration, testosterone hormone, and testicular histology of all groups
were investigated. Expressions of testicular tyrosine phosphorylated and steroidogenic acute regulatory (StAR) proteins were examined
by Western blot. Results showed that MCP contains TPC (39.24±0.65 ug/mg garlic acid), antioxidant capacities (FRAP=33.08±0.21 ug/
mg ascorbic acid equivalent, IC50 of DPPH=389.8±3.20 ug/ml), and momordicine I (404.9 mg/g MCP). Sperm concentration in
MCP80+VPA group was increased as compared to VPA group. Testosterone level in MCP treated groups was significantly increased.
MCP protected testicular damage and could prevent the decrease of StAR and a 50-kDa phosphorylated protein expression in VPAtreated
testis. In conclusion, MCP has antioxidant activities and can prevent male reproductive toxicity in VPA-induced rats.
|
| TH, publication_article_keyword |
Momordica charantia; Momordicine I; Antioxidant capacity; Testicular damage; Valproic acid |
| TH, publication_article_writer |
|
| TH, publication_article_evaluation |
มีผู้ประเมินอิสระ |
| TH, publication_article_status |
ตีพิมพ์แล้ว |
| TH, publication_article_level |
นานาชาติ |
| TH, publication_article_citation |
TH, publication_article_citation_true |
| TH, publication_article_part_of_thesis |
TH, publication_article_part_of_thesis_true |
| TH, publication_article_part_of_graduate |
TH, publication_article_part_of_graduate_false |
| TH, publication_attachment_file |
|
| Citation |
0
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TH, publication_detail
TH, publication_article
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