| Research Title |
Analysis of biomolecular changes in P. insidiosum under exposure to anti – P. insidiosum substances from P. stutzeri and K. pneumoniae using synchrotron radiation based FTIR microspectroscopy |
| Date of Distribution |
5 June 2018 |
| Conference |
| Title of the Conference |
20th Congress International Society for Human and Animal Mycology |
| Organiser |
congress care |
| Conference Place |
RAI Congress Center, Amsterdam, The Netherlands |
| Province/State |
Amsterdam |
| Conference Date |
30 June 2018 |
| To |
4 July 2018 |
| Proceeding Paper |
| Volume |
56 |
| Issue |
2 |
| Page |
143 |
| Editors/edition/publisher |
Karl V. Clemons |
| Abstract |
Objective: Mechanism of action maybe related with biochemical composition (such as lipid, carbohydrate, protein or nucleic acid) changing in P. insidiosum when this organism exposed to the secondary metabolites from P. stutzeri ST1302 and suspected these biomolecules should play a role in the mechanism of inhibitory activity.
Methods: An anti-P. insidiosum substance was extracted from Pseudomonas stutzeri ST1302 by activity guided separation chromatography and confirmed anti-P. insidiosum activity by disc diffusion method. We selected sub-minimal fungicidal concen- tration (MFC) for testing group, 0.02% (wt/vol) thimerosal as positive control and P. insidiosum mycelia fragments in brain heart infusion broth as negative control. The synchrotron radiation-based fourier transform infrared (FTIR) microspectroscopy was used to investigation the biochemical compositions changing in P. insidiosum for each condition.
Results: Crude extract from P. stutzeri ST1302 was separated into 29 fractions by liquid column chromatography. Fraction code number 15 (eluted by 20% MeOH/CH2 Cl2 ) have the best anti-P. insidiosum activity. It showed MFC as 0.625% (wt/vol). For the FTIR measurement, spectra showed significant peak shifts in amide I and amide II regions (1700-1500 cm−1 ) representing alteration of proteins in test groups comparing to control group. Whereas, changes in other biochemical compositions could not be observed.
Conclusion: The in vitro testing of crude extract from P. stutzeri ST1302 showed the anti-P. insidiosum activity by changing some proteins in this organism. Furthermore, the study of proteomics analysis should be done for the better understanding about this mechanism of anti-P. insidiosum activity |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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