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Publication
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| Research Title |
The effects of secreted substance from environmental Klebsiella pneumoniae strain to hyphal form of Pythium insidiosum. |
| Date of Distribution |
23 August 2018 |
| Conference |
| Title of the Conference |
The 9th International Graduate Research Conference |
| Organiser |
Graduate School, Maejo University |
| Conference Place |
The Empress International Convention Center, The Empress Hotel |
| Province/State |
Chiang Mai, Thailand |
| Conference Date |
17 May 2018 |
| To |
18 May 2018 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
103-111 |
| Editors/edition/publisher |
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| Abstract |
Pythium insidiosum is an aquatic fungus like organism, which is the causative agent of
pythiosis, the endemic in Thailand. Because of there is no drug in this treatment for this disease up
to this day, the treatment of choices for pythiosis is surgical removal and most often leads to
disabilities in the affected patients. Thus the search for new compounds for treatment of pythiosis is
still necessary. In our previous study, crude extract from Klebsiella pneumoniae ST2501, the
bacterial isolated from environmental sources shown promising results in antifungal activities
against P. insidiosum. In the present study, the antifungal activities of crude extract from K.
pneumoniae ST2501 and their fractions against 11 clinical P. insidiosum isolates were evaluated
using different end point methods: disc diffusion assay and broth dilution minimal cidal
concentration (MCC) assay. Crude ethyl acetate extract was separated by column chromatography
and subsequently eluted with a gradient of two solvents by gradually increasing the polarity of the
elution solvent system. The eluents were collected and monitored by thin layer chromatography,
producing five groups of eluting fractions. The antifungal activities of crude extract and their
fractions against the mycelia form of P. insidiosum were evaluated using disc diffusion assay (0.5
mg/μl; 20 μl/disc). The crude fraction which shown strong activity was evaluated in quadruplicate
by using broth dilution MCC at the final concentrations ranging from 0.870 to 3.132 mg/ml.
According to the results of disc diffusion assay, crude extract and fraction No. 1 have strong activity
by given the inhibition of 100% of mycelium growth. Crude fraction No. 1 was evaluated antifungal
activities against P. insidiosum by using broth dilution MCC. Seven out of 11 P. insidiosum isolates
were inhibited at 1.566 mg/ml (63.6%); three isolates were inhibited at 1.74 mg/ml (27.3%) while
only one isolate was inhibited at 1.914 mg/ml (9.1%). These results suggested that new compounds
with potential therapeutic activity against pythiosis may come from microorganism or nature.
However, further in vivo studies were needed to validate our findings as well as the acute toxicity
testing in animal models and crude fraction No. 1 could be purified and identified structure in the
near future. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย