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Journal Publication
Research Title IN VITRO ANTIPROLIFERATIVE ACTIVITY AND APOPTOSIS INDUCING ACTIVITY OF RT2, MODIFIED PEPTIDE OF LEUCROCIN I, ON HUMAN COLON CANCER HCT-116 CELLS  
Date of Distribution 20 June 2018 
Conference
     Title of the Conference The 6th International Conference on Biochemistry and Molecular Biology 2018 (BMB 2018) 
     Organiser The Biochemistry and Molecular Section of the Science Society of Thailand and Department of Biochemistry Faculty of science, Chulalongkorn University 
     Conference Place Rayong Resort 
     Province/State Rayong, Thailand 
     Conference Date 20 June 2018 
     To 22 June 2018 
Proceeding Paper
     Volume 2018 
     Issue
     Page 86 
     Editors/edition/publisher  
     Abstract Colon cancer is the third most common cancer causing high mortality rates world-wide. In light of recorded incident, hence, the present study aimed to investigate the effect of RT2, a modified cationic Leucrocin I peptided from Crocodylus siamensis, against HCT-116 human colon cancer cell lines. Using MTT assay, results demonstrated that RT2 at the concentrations of 37.5, 75 and 150 µg/mL exhibited strong cytotoxic effect against HCT-116 cell lines after 24 h of treatment (IC50 = 87.84 µg/mL). This peptide found to be non-toxic to Vero cells. Additionally, RT2 treatment caused a significant reduction in the colony forming ability of cancer cells. A remarkable suppression of cancer cells migration was observed after treatment with RT2, indicating potent anti-metastatic properties. The mechanism involved in cancer cell cytotoxicity of this peptide was shown to be associated with the induction of apoptosis, as evidenced by annexin V-FITC/PI staining flow cytometry analysis. Moreover, the molecular mechanisms of RT2 in apoptosis induction were observed to decrease the mRNA expression levels of Bcl-2 and XIAP and increase expression of Cytochrome C, Caspase-3, and Caspase-9. These collected data clearly demonstrated that RT2 offered great potential in the treatment of human colon cancer cells. 
Author
597020014-0 Mr. SURACHAI MAIJAROEN [Main Author]
Science Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis true 
Presentation awarding false 
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