|
|
Publication
|
| Research Title |
IN VITRO ANTIPROLIFERATIVE ACTIVITY AND APOPTOSIS INDUCING ACTIVITY OF RT2, MODIFIED PEPTIDE OF LEUCROCIN I, ON HUMAN COLON CANCER HCT-116 CELLS |
| Date of Distribution |
20 June 2018 |
| Conference |
| Title of the Conference |
The 6th International Conference on Biochemistry and Molecular Biology 2018 (BMB 2018) |
| Organiser |
The Biochemistry and Molecular Section of the Science Society of Thailand and Department of Biochemistry Faculty of science, Chulalongkorn University |
| Conference Place |
Rayong Resort |
| Province/State |
Rayong, Thailand |
| Conference Date |
20 June 2018 |
| To |
22 June 2018 |
| Proceeding Paper |
| Volume |
2018 |
| Issue |
6 |
| Page |
86 |
| Editors/edition/publisher |
|
| Abstract |
Colon cancer is the third most common cancer causing high mortality rates world-wide. In light of recorded incident, hence, the present study aimed to investigate the effect of RT2, a modified cationic Leucrocin I peptided from Crocodylus siamensis, against HCT-116 human colon cancer cell lines. Using MTT assay, results demonstrated that RT2 at the concentrations of 37.5, 75 and 150 µg/mL exhibited strong cytotoxic effect against HCT-116 cell lines after 24 h of treatment (IC50 = 87.84 µg/mL). This peptide found to be non-toxic to Vero cells. Additionally, RT2 treatment caused a significant reduction in the colony forming ability of cancer cells. A remarkable suppression of cancer cells migration was observed after treatment with RT2, indicating potent anti-metastatic properties. The mechanism involved in cancer cell cytotoxicity of this peptide was shown to be associated with the induction of apoptosis, as evidenced by annexin V-FITC/PI staining flow cytometry analysis. Moreover, the molecular mechanisms of RT2 in apoptosis induction were observed to decrease the mRNA expression levels of Bcl-2 and XIAP and increase expression of Cytochrome C, Caspase-3, and Caspase-9. These collected data clearly demonstrated that RT2 offered great potential in the treatment of human colon cancer cells. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย