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Journal Publication
Research Title The anti-proliferative activity on human colon cancer HCT-116 cells of KT2 and RT2, antimicrobial peptides modified from Siamese crocodile Leucrocin I  
Date of Distribution 7 August 2018 
Conference
     Title of the Conference The 13th International Symposium of the Protein Society of Thailand. 
     Organiser The Protein Society of Thailand (PST) 
     Conference Place The Chulabhorn Research Institute 
     Province/State Bangkok 
     Conference Date 7 August 2018 
     To 9 August 2018 
Proceeding Paper
     Volume 2018 
     Issue 13 
     Page 48 
     Editors/edition/publisher  
     Abstract Colon cancer represents one of the most significantly threat to human health on a global scale and the third and fourth most common cancer in Thai men and women, respectively. Hence, research and development of effective cancer prevention strategies, and these must be clinically effective and display excellent safety in human is in great demand. In light of this challenge, the present study aimed to evaluate the anticancer activity of cationic antimicrobial peptide modified from Leucrocin I (KT2 and RT2) on HCT-116 human colon cancer cell lines. The strong anticancer peptide derived from LL-37 (FK16) and the chemotherapy drug (5-FU) was used as a positive control. The results from MTT assay demonstrated that co-cultured non-cancerous Vero cells with 37.5-75.0 µg/mL peptides (KT2, RT2 and FK16) for 3, 6, 12, and 24 h had no effect on the viability of the cells. Considering 5-FU, a slight reduction in cell viability could be observed from the concentration of 37.5 µg/mL, after 6 h treatment. The ability of all peptides to inhibit the growth of HCT-116 cells could be significantly from 3 h of treatment. Conversely, 5-FU exhibited significant inhibition of the growth of the HCT-116 cells from 6 h of treatment. The IC50 values of KT2 for 12 and 24 h treatments were 111.96 and 90.25 µg/mL, respectively. On the contrary, IC50 values of 104.07 and 87.84 µg/mL were observed when HCT-116 cells were treated with RT2 for 12 and 24 h, respectively. Additionally, the colony count indicated that peptides (KT2, RT2 and FK16) induced a dose-dependent decrease in the ability of colony formation of HCT-116 cells.  
Author
597020014-0 Mr. SURACHAI MAIJAROEN [Main Author]
Science Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis true 
Presentation awarding false 
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