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Publication
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| Title of Article |
Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B1 contamination in dairy cattle feed |
| Date of Acceptance |
26 July 2018 |
| Journal |
| Title of Journal |
Journal of Applied Microbiology |
| Standard |
ISI |
| Institute of Journal |
John Wiley & Sons, Ltd. |
| ISBN/ISSN |
1364-5072 |
| Volume |
2018 |
| Issue |
125 |
| Month |
12 |
| Year of Publication |
2018 |
| Page |
1603-1613 |
| Abstract |
Aims: To obtain yeast and bacteria from ruminal fluids that possess aflatoxin B1 (AFB1) detoxifying ability for use in animal feed.
Methods and Results: Sources of isolation were ruminal fluids of three non–fistulated dairy cows, fed with diets containing cassava pulp, rice straw, or distillery yeast sludge. The isolation was carried out to screen for the isolates that were active in both anaerobic and aerobic conditions. Three yeast and 3 bacteria isolates were selected. Up to 85% of AFB1 was detoxified by yeast isolates and up to 60% AFB1 reduction was evident by bacteria isolates. Two yeast isolates were identified as Kluyveromyces marxianus and 1 isolate as Pichia kudriavzevii. The 3 bacteria isolates were identified as Enterococcus faecium, Corynebacterium phoceense and C. vitaeruminis. All strains showed high biomass production when cultivated in medium with 80 g l-1 glucose.
Conclusions: The isolated yeast and bacteria with AFB1-detoxifying ability showed a good potential to be applied as an aflatoxin-detoxifying agent to ingredients used to feed dairy cattle.
Significance and Impact of Study: The abilities of isolates to survive and active in anaerobic and aerobic conditions rendered them to be active in cattle’s rumen. Their biomass could be produced in bulk and used as feed supplement for the purpose of aflatoxins detoxification in dairy cattle. |
| Keyword |
aflatoxin B1, dairy cattle, isolation, detoxify, yeast, bacteria |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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