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Publication
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| Research Title |
FT-IR Microspectroscopy analysis of Beta-Glucan structure from spent yeast Saccharomyces cerevisiae TISTR 5339 |
| Date of Distribution |
18 October 2018 |
| Conference |
| Title of the Conference |
NATPRO 7 |
| Organiser |
ASNP The Asian Society of Natural Product |
| Conference Place |
Hotel Hyundai |
| Province/State |
Gyeongju, Korea |
| Conference Date |
18 October 2018 |
| To |
20 October 2018 |
| Proceeding Paper |
| Volume |
7 |
| Issue |
1 |
| Page |
84 |
| Editors/edition/publisher |
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| Abstract |
Beta-glucans are linear chain of glucose residues polymerized through β-1,3 β-1,4 or β-1,6 glycosidic linkages. Difference structures of beta-glucans found depend on source of beta-glucans. Yeast biomass remaining in the reactor after bioethanol fermentation process is an important source of beta-1,3/1,6-glucan which has several benefits on biological response, especially its function as immunostimulants to protect cells against infectious disease is from beta-1,3-glucan. In this study, Saccharomyces cerevisiae TISTR 5339 biomass was disrupted with three different methods: autolysis, sonication, and liquid nitrogen. Moreover, extracted yeast beta-glucans were subjected to the fourier-transform infrared (FTIR) spectroscopy for composition analysis. The result showed that the polysaccharide content from three disruption methods were 64.9%, 60.1%, 52.5% from autolysis, sonication, and liquid nitrogen, respectively. FTIR spectrum indicated that the sample derived from autolysis disruption method provided the highest content of beta-1,3-glucan of total polysaccharide. Therefore, autolysis is the recommended method for spent yeast cells disruption to obtain highest beta-1,3-glucan after extraction process. |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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