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Journal Publication
Research Title FT-IR Microspectroscopy analysis of Beta-Glucan structure from spent yeast Saccharomyces cerevisiae TISTR 5339 
Date of Distribution 18 October 2018 
Conference
     Title of the Conference NATPRO 7 
     Organiser ASNP The Asian Society of Natural Product 
     Conference Place Hotel Hyundai 
     Province/State Gyeongju, Korea 
     Conference Date 18 October 2018 
     To 20 October 2018 
Proceeding Paper
     Volume
     Issue
     Page 84 
     Editors/edition/publisher  
     Abstract Beta-glucans are linear chain of glucose residues polymerized through β-1,3 β-1,4 or β-1,6 glycosidic linkages. Difference structures of beta-glucans found depend on source of beta-glucans. Yeast biomass remaining in the reactor after bioethanol fermentation process is an important source of beta-1,3/1,6-glucan which has several benefits on biological response, especially its function as immunostimulants to protect cells against infectious disease is from beta-1,3-glucan. In this study, Saccharomyces cerevisiae TISTR 5339 biomass was disrupted with three different methods: autolysis, sonication, and liquid nitrogen. Moreover, extracted yeast beta-glucans were subjected to the fourier-transform infrared (FTIR) spectroscopy for composition analysis. The result showed that the polysaccharide content from three disruption methods were 64.9%, 60.1%, 52.5% from autolysis, sonication, and liquid nitrogen, respectively. FTIR spectrum indicated that the sample derived from autolysis disruption method provided the highest content of beta-1,3-glucan of total polysaccharide. Therefore, autolysis is the recommended method for spent yeast cells disruption to obtain highest beta-1,3-glucan after extraction process.  
Author
595160021-8 Mr. RAKSMEY THIN [Main Author]
Technology Master's Degree
547160004-7 Miss ATIYA TECHAPARIN
Technology Doctoral Degree

Peer Review Status ไม่มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Oral 
Part of thesis true 
Presentation awarding false 
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