| Research Title |
Anisotropic Silver Nanoparticles, an Alternative Treatment as the Effective Antimicrobial Agents |
| Date of Distribution |
12 December 2018 |
| Conference |
| Title of the Conference |
The 6th Thailand International Nanotechnology Conference 2018 |
| Organiser |
National Nanotechnology Center (NANOTEC) |
| Conference Place |
Thailand Science Park Convention Center Prathum Thani, Thailand |
| Province/State |
ปทุมธานี |
| Conference Date |
12 December 2018 |
| To |
14 December 2018 |
| Proceeding Paper |
| Volume |
2016 |
| Issue |
1 |
| Page |
231 |
| Editors/edition/publisher |
|
| Abstract |
Silver nanoparticles (AgNPs) are known as the effective antibacterial agent. The antimicrobial effect
of AgNPs depends on physical properties including their shape and size. In this research, we
evaluated antibacterial activity and cytotoxic effect of both AgNPs and anisotropic AgNPs which
have difference in size and shape. The AgNPs were synthesized by chemical method and the
colloidal solution showed yellow color then such AgNPs were added into H2O2 to obtain
anisotropic AgNPs. The result from characterization by TEM found that AgNPs with yellow solution
showed average sizes of 5 nm in spherical shape while anisotropic AgNPs with orange solution
showed average sizes of 30 nm in mix shapes of spherical, triangular and hexagonal nanoplates. The
antibacterial activity by the microdilution plating method found that minimum inhibitory concentration
(MIC) and minimum bactericidal concentration (MBC) values of AnNPs against E.coli O157/H7
and S.aureus ATCC25923 are in the range of 1-8 μg/mL and 2-16 μg/mL, repectively. In a similar
way, the MIC and MBC value of anisotropic AgNPs against E.coli O157:H7 and S.aureus ATCC
25923 are in the range of 2-16 μg/mL and 2-32 μg/mL, repectively. For the cytotoxicity, the result
exhibited that AgNPs showed an IC50 of 30.80±14.67 μg/mL and 17.70±13.67 μg/mL on cell line
of keratinocyte and fibroblast while anisotropic AgNPs showed an IC50 of 65.39±13.84 μg/mL and
65.39±13.84 μg/mL in the same cell with AgNPs testing. From these results reveal that antimicrobial
activity of AgNPs and anisotropic AgNPs is slightly different but cytotoxic effect of AgNPs show
more cytotoxic than anisotropic AgNPs in both types of cell line. These finding suggest that both
AgNPs types is efficient antimicrobial agent against bacteria, especially anisotropic AgNPs is safer
for using in living organisms. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
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Publication
Journal Publication
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