| Research Title |
Effect of Xenobiotics on the Expression of CYP450s and Transporter in HepG2 Cells |
| Date of Distribution |
16 March 2019 |
| Conference |
| Title of the Conference |
The 11th Annual Conference of Northeast Pharmacy Research 2019 |
| Organiser |
Mahasarakham University, Khon Kaen University, UbonRatchathani University |
| Conference Place |
Faculty of Pharmacy, Mahasarakham University |
| Province/State |
Mahasarakham |
| Conference Date |
16 March 2019 |
| To |
17 March 2019 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
17 |
| Editors/edition/publisher |
- |
| Abstract |
Introduction: Nicotine in tobacco/cigarette smoke is highly addictive and N-Nitrosodiethanolamine (NDELA) is one of the most common nitrosamines, also found in tobacco products, which are activated within the body to form tumors both locally and systemically. CYP1A2 and CYP2B6 especially expressed in liver are considered to be of particular importance for nicotine and NDELA biotransformation. In addition, OATP1B1 are responsible for the cellular uptake of a broad range of xenobiotics in liver tissues. The aim of the current study was to determine nicotine and NDELA-mediated modulation in the expression of CYP1A2, CYP2B6 and OATP1B1 using human liver hepatoma derived cell line (HepG2). Materials and methods: HepG2 cells (5×105 cells in a 6-well plate) were treated with nicotine and NDELA (1μM, 10μM, and 100μM) for 24h. Total RNA was isolated using guanidinium-thiocyanate-phenol-chloroform method, and cDNA was reverse-transcribed. The mRNA expression was analyzed by qPCR using specific primers and SYBR green, compared to that of GAPDH (a reference gene). Results: Nicotine at the concentration range studied (1-100μM) caused a concentration-dependent induction of CYP1A2 mRNA, but this induction was slightly lower than that of NDELA. CYP2B6 was induced significantly (10-fold) by high nicotine concentration and 15-fold by high NDELA concentration. Three types of concentration for both nicotine and NDELA affected the mRNA level of OATP1B1 induction significantly. Conclusion: Nicotine and NDELA induce mRNA expression level of CYP1A2, CYP2B6 and OATP1B1 in vitro significantly. Therefore, smokers might have a high potential for interaction with concurrently administered CYP1A2, CYP2B6 and OATP1B1 substrates. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
Very good oral presentation |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
Mahasarakham University, Khon Kaen University, UbonRatchathani University |
| Date of awarding |
17 มีนาคม 2562 |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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