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Publication
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| Title of Article |
Antitumor effects of candidone extracted from Derris indica (Lamk) Bennet in cholangiocarcinoma cells |
| Date of Acceptance |
23 June 2018 |
| Journal |
| Title of Journal |
Tropical Journal of Pharmaceutical Research |
| Standard |
SCOPUS |
| Institute of Journal |
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin,Nigeria |
| ISBN/ISSN |
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| Volume |
17 |
| Issue |
7 |
| Month |
July |
| Year of Publication |
2019 |
| Page |
1337-1343 |
| Abstract |
Purpose: To investigate the antitumor effect of candidone extracted from Derris indica, against human
cholangiocarcinoma (CCA) cells.
Methods: Candidone was purified from the hexane extract of Derris indica fruit. CCA cell lines, KKUM156
and KKU-M213, were treated with candidone. Sulforhodamine B (SRB) assay and acridine
orange/ethidium bromide (AO/EB) staining were used to investigate the effects of candidone on cell
proliferation and induction of apoptosis, respectively. The effect on cell migration was assessed by
wound healing assay. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was
employed to assess the effects of candidone on the expression of genes that regulate proliferation and
apoptosis.
Results: Candidone exerted strong anticancer effects on CCA cells. The agent suppressed CCA cell
proliferation and induced apoptotic cell death. RT-qPCR assay revealed that candidone significantly
increased the expression of anti-proliferative and pro-apoptotic genes, including p21 and Bax, and
decreased the expression of anti-apoptotic genes, including Bcl-2 and survivin. Moreover, candidone
inhibited the migration of CCA cells induced by IGF-1.
Conclusion: Candidone exhibits potent antitumor effect on CCA cells. These findings suggest that
candidone is potentially suitable for the management of CCA and, therefore, warrants further
investigation. |
| Keyword |
Candidone, Derris indica, Cholangiocarcinoma, Cytotoxicity, Apoptosis |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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