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Publication
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| Title of Article |
Gold nanorods enhanced resonance Rayleigh scattering for detection
of Hg2+ by in-situ mixing with single-stranded DNA |
| Date of Acceptance |
16 August 2017 |
| Journal |
| Title of Journal |
Sensors and Actuators B: Chemical |
| Standard |
SCOPUS |
| Institute of Journal |
ELSEVIER |
| ISBN/ISSN |
http://dx.doi.org/10.1016/j.snb.2017.08.129 |
| Volume |
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| Issue |
255 |
| Month |
February |
| Year of Publication |
2019 |
| Page |
836-842 |
| Abstract |
A resonance Rayleigh scattering (RRS) method for determination of Hg2+ concentration using positively charged gold nanorods (GNRs) and thymine-rich single-stranded DNA (ssDNA) was developed. Hg2+ can induce a conformation change of ssDNA from a random coil to a hairpin structure via thymine–Hg2+–thymine coordination. Hairpin shaped DNA possesses a high density of negative charges, therefore it caused in a drastic aggregation of GNRs. This resulted in an enhancement of RRS intensity. In this work, a change in RRS intensity proportional to the concentration of Hg2+ was monitored by spectrofluorometer at excitation and RRS wavelengths of 550 nm (λex = λRRS). This method offered a detection limit of 0.23 nM, which is lower than the maximum mercury contaminant level specified by the US Environmental Protection Agency. Good precision of analysis was obtained (3.1% relative standard deviation). Furthermore, the method was simple, involving in-situ mixing and sensing in a one-pot solution. Detection was accomplished in 5 min. This makes the method advantageous in terms of convenience and rapidity of analysis. The developed method was successfully applied for detection of Hg2+ in tap water samples. Recoveries were found in range of 100.6–119.0%. |
| Keyword |
Aggregation, Gold nanorods, Hg2+, Resonance Rayleigh scattering, ssDNA, Tap water |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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