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Publication
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| Research Title |
Effect of gold nanoparticles with different surface charges on cellular internalization and cytokine response in monocytes |
| Date of Distribution |
6 December 2018 |
| Conference |
| Title of the Conference |
4th Pan-Asian Biomedical Science Conference |
| Organiser |
the University of Danang and the Asia International Science Consortium |
| Conference Place |
Furama Resort, Danang, Vietnam |
| Province/State |
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| Conference Date |
6 December 2018 |
| To |
7 December 2018 |
| Proceeding Paper |
| Volume |
4 |
| Issue |
4 |
| Page |
166-167 |
| Editors/edition/publisher |
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| Abstract |
Gold nanoparticles (AuNPs) is growing up to use in biomedicine due to their ease of fabrication, multiple surface functionalities and being inert. In the applications for biomedicine, it might be concerned about cellular interactions and consequences related to biological system, especially in a part of innate immunity as phagocytic functions, which are the earliest line of defense, need to be clarified. Thus, an investigation into how different surface charges, but on AuNPs with similar sizes and shapes, affected the internalization mechanisms and immune functions of monocytes is needed. Here, the U937 human monocyte cell line was used to investigate the cellular responses to the different surface charges on AuNPs. The AuNPs with the different charges entering the cells were investigated by ICP-MS and TEM. The results revealed that positively charged AuNPs (AuNPs+ve) might be entered through macropinocytosis, whereas the negatively charged AuNPs (AuNPs-ve) internalization has been unclear. However, the results indicted different responses to AuNP+ve and AuNPs-ve, including cell activation and cytokine productions. The AuNPs+ve significantly enhanced the mRNA expression of IL-1β and TGF-β by 1.28 and 1.1-folds respectively, while AuNPs-ve stimulated TNF-α expression by 1.27-folds, compared to control. Moreover, AuNPs+ve strongly provoked a cellular response to increase IL-6 production through NF-kB expression by 3-folds while AuNPs-ve significantly decreased the expression by 5-folds compared to the control, respectively. Therefore, these results provide essential information for designing nanoparticles with possible applications in nanomedicine, including drug delivery and vaccine development in the future. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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