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Publication
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| Research Title |
Construction and expression of dengue nonstructural 1 (NS1) protein in Lactobacillus casei |
| Date of Distribution |
7 August 2019 |
| Conference |
| Title of the Conference |
Asian Neglected Tropical Disease Conference (NTDASIA) 2019 |
| Organiser |
Tropical Disease Research Laboratory, Department of Pathology, Medicine, Khon Kaen University, THAILAND |
| Conference Place |
Pullman Khon Kaen Raja Orchid, Khon Kaen, Thailand |
| Province/State |
Khonkaen |
| Conference Date |
7 August 2019 |
| To |
9 August 2019 |
| Proceeding Paper |
| Volume |
2019 |
| Issue |
1 |
| Page |
87-91 |
| Editors/edition/publisher |
Yukifumi Nawa, Ted (Chia-Kwung) Fan, Sung-Jung Hong, Ayako Yoshida, John F. Smith, Prasarn tangkawattana, Thewarach laha, Kanin salao, Sutas suttiprapa, Prasert saichua, Nantana suwandittakul |
| Abstract |
Dengue nonstructural 1 (NS1) is one of an attractive candidate antigen to be incorporated in dengue vaccine due to its ability to elicit protective immunity that can cross-react with all types of dengue virus. In this study, the codon-optimized NS1 gene was cloned in our Escherichia coli-L.casei shuttle vector using a constitutive promoter of lactate dehydrogenase gene, pRCEID-LC13.9. The two recombinant plasmid containing NS1 gene, pRCEID-LC13.9:NS1 without signal peptide and pRCEID-LC13.9:USP:NS1 carried the signal peptide, Usp45, were constructed and were each transformed in the expression host, L. casei RCEID02. It was found that the recombinant L. casei carrying pRCEID-LC13.9:NS1 and that carrying pRCEID-LC13.9:USP:NS1 can successfully expressed NS1 protein as intracellular and secreted form as determined by immunoblotting using anti-NS1 antibody. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย