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Publication
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| Research Title |
Effect of Nanomaterial Properties on Cellular Immune Responses: Focusing on Gold Nanoparticles |
| Date of Distribution |
27 September 2019 |
| Conference |
| Title of the Conference |
the LMCE 2019 (Laboratory Medicine Congress & Exhibition) & KSLM 60th Annual Meeting |
| Organiser |
Korean Society for Laboratory Medicine |
| Conference Place |
BEXCO |
| Province/State |
Busan, Korea |
| Conference Date |
26 September 2019 |
| To |
28 August 2019 |
| Proceeding Paper |
| Volume |
39 |
| Issue |
1 |
| Page |
S14 |
| Editors/edition/publisher |
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| Abstract |
Background: In recent years, nanomaterial especially gold nanoparticles (AuNPs) is used in a wide range of biomedical applications due to their excellent properties, high compatibility with tissue and multiple surface functionalities. However, cellular responses and consequences related to biological system, especially phagocytic functions, which are the earliest line of defense in immune system, need to be illustrated. Thus, an investigation into how different surface charges, but on AuNPs with similar sizes and shapes, affected the internalization mechanisms and immune functions of monocytes is needed.
Methods: The human monocytes cell line U937 was incubated in cell culture media containing the positively charged AuNPs (AuNPs+ve) and negatively charged AuNPs (AuNPs-ve) to investigate the cellular responses. The AuNPs with the different charges entering the cells were investigated by ICP-MS and TEM. Cell activations and cytokine productions were examined by western blot and RT-PCR.
Results: The results revealed that the AuNPs+ve entered cells through macropinocytosis and strongly activated cells to produce IL-1β, TGF-β and IL-6 by enhancing NF-kB expression whereas cellular uptake of AuNPs-ve might be taken up via a mechanism involving ATP, which tended to be clathrin-mediated endocytosis (CME) and suppressed NF-kB expression leading to reduce IL-6 production.
Conclusions: Therefore, these results provided the essential information including internalization and immunomodulation that could be applied in nanomedicine for designing and developing drug delivery system, vaccine and medical device in the future.
Key words: Immunity, Surface charge, Gold nanoparticles, Internalization, Cytokine
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
Travel grant award |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
the LMCE 2019 (Laboratory Medicine Congress & Exhibition) & KSLM 60th Annual Meeting |
| Date of awarding |
27 กันยายน 2562 |
| Attach file |
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| Citation |
0
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Journal Publication
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