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Publication
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| Title of Article |
Different concentrations of Cysteamine, Ergothioneine, and Serine modulate quality and fertilizing ability of cryopreserved chicken sperm |
| Date of Acceptance |
11 October 2019 |
| Journal |
| Title of Journal |
Poultry Science |
| Standard |
SCOPUS |
| Institute of Journal |
Oxford University Press (OUP) on behalf of the Poultry Science Association, Inc. |
| ISBN/ISSN |
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| Volume |
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| Year of Publication |
2019 |
| Page |
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| Abstract |
In order to optimize chicken sperm cryopreservation, the aim of this study was to evaluate the effects of freezing diluents supplementation in three potential amines/amino acids antioxidant cysteamine (2-amino ethanethiol, AET), ergothioneine (ERG), and serine (SER). The semen of thirty-six Pradu Hang Dum males, selected on their motility vigor score, was frozen with a simple freezing method using nitrogen vapors and dimethylformamide (DMF). In a first experiment, a wide range of AET, ERG, and SER doses was tested. Semen quality was evaluated after incubation at 5°C or after cryopreservation in straws in the Blumberger hahnen sperma-verdünner (BHSV) diluent + DMF (6% v/v) with or without AET/ERG/SER. The best targeted doses of AET/ERG/SER were then selected for experiment 2 that was focused on cryopreserved semen. Frozen-thawed sperm quality was evaluated by different in vitro tests and by evaluation of fertility. Objective motility parameters were evaluated by CASA. Membrane integrity, acrosome integrity, mitochondria function were evaluated using appropriate dyes and flow cytometry. Lipid peroxide production was assessed by the thiobarbituric acid test (Malondialdehyde production). Fertility obtained with frozen-thawed semen supplemented or not in AET, ERG, or SER was evaluated after artificial insemination of laying hens. ERG and AET decreased sperm lipid peroxidation but decreased fertility, even at low doses. The presence of 4 mmol SER significantly decreased lipid peroxidation, increased the frozen-thawed sperm quality, and increased fertility after sperm cryopreservation (90% vs control 84%, P<0.05). In a third experiment, the use of 1 mmol sucrose (SU) (the best result of our previous study) added to 4 mmol SER supplemented extender was tested. This addition allowed to the highest levels of fertility (93%). In conclusion, the addition of 4 mmol SER in semen cryopreservation diluents decreases peroxidation and improves the efficiency of the process. |
| Keyword |
serine, semen cryopreservation, chicken, fertility |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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