| Research Title |
Differential impacts of phenol red on rifampicin-induced expression of cytochrome P450 mRNAs between HepG2 and Caco-2 models |
| Date of Distribution |
21 November 2019 |
| Conference |
| Title of the Conference |
ประชุมวิชาการ เนื่องในวันสถาปนาคณะแพทยศาสตร์ มหาวิทยาลัยมหาสารคาม ปีที่ 16 |
| Organiser |
คณะแพทยศาสตร์ มหาวิทยาลัยมหาสารคาม |
| Conference Place |
ห้องประชุมเฉลิม วราวิทย์ อาคารศูนย์บริการทางการแพทย์และศูนย์วิจัยเฉลิมพระเกียรติ หลังที่ 2 คณะแพทยศาสตร์ มหาวิทยาลัยมหาสารคาม |
| Province/State |
มหาสารคาม |
| Conference Date |
21 November 2019 |
| To |
21 November 2019 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
53 |
| Editors/edition/publisher |
- |
| Abstract |
Introduction: Phenol red is a pH indicator to detect a change of culture medium from neutral to acidic condition but how phenol red influences the expression of cytochrome P450 enzymes (CYP) in a cell culture is limited. Hepatocarcinoma (HepG2) and adenocarcinoma (Caco-2) cells are widely used as the representative models for human liver and intestine, the major organs of drug metabolism particularly for oral drugs. Objective: To examine effects of phenol red in the culture media on profiles of CYP1A2, CYP2E1, CYP3A4, and CYP3A5 mRNAs in HepG2 and Caco-2 cells induced by rifampicin. Materials and methods: HepG2 (1.25×105 cells/well) and Caco-2 (5×104 cells/well) were cultured in a 24-well plate for 72 h. Then the cells were treated with 5 M rifampicin in phenol red or non-phenol red contained culture media for 72 h. The expressions of CYP1A2, CYP2E1, CYP3A4, and CYP3A5 mRNAs were quantitatively determined by RT/qPCR. Results: Rifampicin significantly induced the expressions of CYP3A4 and CYP3A5 in HepG2 in non-phenol red contained medium while the expressions of CYP1A2 and CYP2E1 in HepG2 were similar in both mediums. Regarding Caco-2, rifampicin significantly induced all CYPs in phenol red contained medium while the induction of CYPs by rifampicin was very low in non-phenol red contained medium. Discussion and Conclusion: Phenol red is one factor affecting the induction of CYPs. Besides, the presence of phenol red leads to the different results depending on the cell lines. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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|
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Publication
Journal Publication
ไทย