| Abstract |
A simple and highly sensitive method is developed for the simultaneous determination of Ni2+, Cr2O72−, Co2+, and Hg2+ by using in syringe low density solvent-dispersive liquid liquid microextraction (ISLD-DLLME), followed by high performance liquid chromatography with a UV
detector. The four metal ions were derivatized with pyrrolidine dithiocarbamate (PDC) based on complexation before their enrichment by ISLD-DLLME in which 1-octanol and methanol were
used as the extraction solvent and the dispersive solvent, respectively. The extraction was
performed in a commercially available syringe under vortex agitation. Phase separation was achieved without centrifugation, and the extraction phase was easily collected by moving the syringe plunger. Parameters affecting the extraction efficiency were studied and optimized. Under
the optimum conditions, the four metal-PDC complexes were detected within 18 min, and ISLDDLLME
could increase the detection sensitivity in the range of 64–230 times compared to the direct HPLC analysis. The obtained limits of detection (LODs) were found to be in the range of 0.011–2.0 μg L−1. The applicability of the method is demonstrated for freshwater fish, shrimp, and
shellfish samples. In addition, the results are in good agreement with those obtained by inductively-coupled plasma-optical emission spectrometry (ICP-OES). |
Publication
Journal Publication
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