|
|
Publication
|
| Research Title |
Evaluation of the efficacy of disinfectant solutions against cyst of Acanthamoeba species, Thailand isolates. |
| Date of Distribution |
21 November 2019 |
| Conference |
| Title of the Conference |
International Conference on Parasitology 2019 Theme: Innovative Research in Parasitology. |
| Organiser |
Department of Parasitology, Faculty of Medicine, Khon Kaen University |
| Conference Place |
Northeastern Science Park, Khon Kaen University, Khon Kaen, Thailand |
| Province/State |
Khon Kaen |
| Conference Date |
21 November 2019 |
| To |
22 November 2019 |
| Proceeding Paper |
| Volume |
2019 |
| Issue |
1 |
| Page |
46-52 |
| Editors/edition/publisher |
|
| Abstract |
The present study aimed to investigate the efficacy of disinfectant solutions that
commonly used in hospitals against Acanthamoebae cysts (genotypes T3, T4 and T5). After incubation with disinfectant, the cyst viability was examined by staining with 0.4% trypan blue.The result showed that treatment with 1%Virkon® was efficient against T4 and T5 genotypes for 10 min. Whereas T3 genotype had to use 2% Virkon® for inactivation. In this study, Clorox® solution with 2.5% and 6% sodium hypochlorite was not efficient. Exposure to 70% ethanol for 30 min was effective for T4 and T5 but not effective for T3, whereas 95% ethanol can kill all genotypes for 30 min. The results showed that T3 genotype is more resistance than the other
two genotypes (P< 0.0001) and 2% Virkon® had highly effective (P< 0.005). The viability and
non-viability after treatments were determined by staining with 0.4% trypan blue and confirmed
by NNA (non-nutrient agar) culture. For heating, all genotypes were not completely inactivated
at 55°C, whereas exposure at 65 °C did result in inactivation. Effective disinfection is important
not only to prevent the transmission of Acanthamoeba itself but also to reduce the risks of
infection with other microbial pathogens that are associated intracellularly with Acanthamoeba. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย