2012 ©
             Publication
Journal Publication
Research Title Effects of phenol red on the induction of metabolism- and transportation- related genes by rifampicin in HepG2 and Caco-2 cells 
Date of Distribution 15 February 2020 
Conference
     Title of the Conference The 12th Annual Conference of Northeastern Pharmacy Research 2020: Transformation of Pharmacy Profession in The Digital Era 
     Organiser คณะเภสัชศาสตร์ มหาวิทยาลัยอุบลราชธานี มหาวิทยาลัยขอนแก่น มหาวิทยาลัยมหาสารคาม 
     Conference Place คณะเภสัชศาสตร์ มหาวิทยาลัยอุบลราชธานี 
     Province/State อุบลราชธานี 
     Conference Date 15 February 2020 
     To 16 February 2020 
Proceeding Paper
     Volume
     Issue
     Page 59 
     Editors/edition/publisher
     Abstract Introduction: Phenol red is commonly used as the indicator for monitoring pH change in cell culture media. However, whether phenol red interferes the induction and expression of cytochrome P450 (CYPs) and drug transporters in cell culture models are still limited. Rifampicin is the standard anti-tuberculosis drug that normally used as the keystone for metabolizing enzyme studies because of board spectrum CYPs induction property. Objective: To examine the effects of phenol red in culture media on the expression of drug metabolism- and transportation-related genes, including CYP3A4, CYP3A5, solute carrier organic anion transporter family member 1B1 (SLCO1B1), and pregnane X receptor (PXR), in human hepatocarcinoma (HepG2) and human colon adenocarcinoma (Caco-2) cells. Methods: HepG2 (1.25x105 cells/well) and Caco-2 (5x104 cells/well) were cultured in a 24-well plate for 72 h before treated with 5 µM rifampicin in phenol red or non-phenol red contained culture media, for the next 72 h. The expressions of CYP3A4, CYP3A5, SLCO1B1, and PXR mRNAs were quantitatively determined by RT/qPCR. Results: In HepG2 cells, CYP3A4, CYP3A5, and SLCO1B1 mRNAs were significantly increased by rifampicin in both of phenol red and non-phenol red contained medium (p<0.05). Interestingly, non-phenol red contained medium showed the higher expression of those genes than phenol red contained medium (p<0.05). On the other hand, PXR was decreased in phenol red contained medium but it was unchanged in non-phenol red contained medium. In Caco-2 cells, rifampicin significantly elevated the expression of CYP3A4, CYP3A5, and SLCO1B1 in non-phenol red contained medium (p<0.05) while the expression of aforementioned genes was not modified by rifampicin in phenol red contained medium. In addition, PXR mRNA was not altered by any treatments. Conclusion: Phenol red is a crucial factor that might suppress the regulatory mechanism of CYPs, drug transporters, and nuclear receptors. Phenol red affects the induction of CYPs (CYP3A4 and CYP3A5), transporter (SLCO1B1), and nuclear receptor (PXR) differently between HepG2 and Caco-2 cells. Hence, phenol red contained medium should be employed with a strong concern for drug interaction prediction in a cell line model. 
Author
625150002-1 Mr. NATHAPHON KUNCHAROENWIRAT [Main Author]
Pharmaceutical Sciences Master's Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference ชาติ 
Type of Proceeding Abstract 
Type of Presentation Oral 
Part of thesis true 
Presentation awarding true 
     Award Title ดีมาก ในาการนำเสนอผลงานประเภท Oral Presentation ระดับบัณฑิตศึกษาและบุคคลทั่วไป ด้านวิทยาศาสตร์เภสัชกรรม 
     Type of award รางวัลด้านวิชาการ วิชาชีพ 
     Organiser คณะเภสัชศาสตร์ มหาวิทยาลัยอุบลราชธานี 
     Date of awarding 16 กุมภาพันธ์ 2563 
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