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Publication
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| Title of Article |
Optimization of Culture Conditions for Xylanase Production
from Cellulase-free Xylanase-producing Thermophilic
Fungus, Thermomyces dupontii KKU−CLD−E2−3 |
| Date of Acceptance |
23 December 2019 |
| Journal |
| Title of Journal |
Chiang Mai J. Sci. |
| Standard |
SCOPUS |
| Institute of Journal |
Faculty of Science, Chiangmai University |
| ISBN/ISSN |
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| Volume |
2020 |
| Issue |
47 |
| Month |
May |
| Year of Publication |
2020 |
| Page |
391-402 |
| Abstract |
The thermoalkaliphilic cellulase-free xylanase-producing fungi, Thermomyces dupontii KKU−CLD−
E2−3 was isolated from elephant dung. The production of thermo-alkali-stable and cellulase-free xylanase
of the fungus was optimized in solid-state fermentation using statistical approaches. Response surface
methodology (RSM) using central composite design (CCD) was employed to optimize conditions. The
maximum xylanase production was observed at a 1.72% nitrogen concentration, initial pH of 10.54, initial
moisture content of 74.73% and under incubation for 8 days at a temperature of 43.93 oC. The maximum
xylanase activity was evaluated at pH 11.0 and retained over 80% of the original activity within a pH range
of 7.0–10.0 after incubation at 4 oC for 24 h. In addition, the optimum temperature for xylanase activity
was 80 oC. The enzyme also retained more than 80% of its residual activity after heated at 60 oC for 90 min.
Therefore, its properties demonstrate significant promise for use in pulp bleaching for the paper industry;
for which this study will be the focus of future investigation. |
| Keyword |
elephant dung, thermo-alkaliphilic fungi, response surface methodology |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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