| Research Title |
Use of human antimicrobial peptides to combat of multidrug-resistant uropathogenic Escherichia coli causing catheter associated urinary tract infections |
| Date of Distribution |
7 December 2019 |
| Conference |
| Title of the Conference |
The 49th National Graduate Research Conference : NGRC |
| Organiser |
Council of the Graduate Studies Administrators of Thailand (CGAT) |
| Conference Place |
Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat, Thailand |
| Province/State |
Nakhon Si Thammarat, Thailand |
| Conference Date |
6 December 2019 |
| To |
7 December 2019 |
| Proceeding Paper |
| Volume |
2562 |
| Issue |
1 |
| Page |
199-210 |
| Editors/edition/publisher |
Asst. Prof. Dr. LADAWAN KAEWSEENUAL |
| Abstract |
Catheter associated urinary tract infections or CAUTIs are one common type of healthcare associated infections. Uropathogenic Escherichia coli (UPEC) plays the leading role in the pathogenesis of CAUTIs, the main issue that make CAUTIs harder to treat is the increasing of drug-resistant incidence. Therefore, novel antimicrobial agent such as cathelicidin antimicrobial peptide (CAMP) was investigated. The aim of this study was to determine the antimicrobial activity of human CAMP (LL-37), its truncated peptide (LL-31) and D-enantiomer form (D-LL-37, D-LL-31, D-LL-13-31 and D-LL-13-37) against clinical isolates of multidrug-resistant (MDR)-UPEC. Ten MDR-UPEC clinical isolates were collected from patients presented with catheter associated urinary tract infections. Antimicrobial susceptibility of all isolates were tested using micro-broth dilution technique and antimicrobial activity of all peptides and time-kill kinetics of the most effective peptide against MDR-UPEC in planktonic form were determined using colony counting assay. The minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) of ceftriaxone against all MDR-UPEC isolates in planktonic form were equal or greater than 1,024 μg/ml. While 20 μM of LL-37, D-LL-37, LL-31 and D-LL-31 exhibited 100% killing activity against MDR-UPEC within 1 hour. Killing kinetics revealed that 1 μM of D-LL-31 displayed the strongest bactericidal activity which can reach the bacterial endpoint within 1 hour against highly resistant isolate, while D-LL-37 which required 24 hours. Moreover, conventional antibiotic ceftriaxone had no killing antimicrobial effect in higher concentration (15 μM) along 24 hours of incubation period. These results indicate that D-LL-31 might be possible to develop as novel antimicrobial agent against CAUTIs in the future. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย