2012 ©
             Publication
Journal Publication
Title of Article Multitarget Activities of Kleeb Bua Daeng, a Thai Traditional Herbal Formula, Against Alzheimer’s Disease 
Date of Acceptance 23 April 2020 
Journal
     Title of Journal Pharmaceuticals 
     Standard SCOPUS 
     Institute of Journal Multidisciplinary Digital Publishing Institute (MDPI) 
     ISBN/ISSN 1424-8247 
     Volume 13 
     Issue
     Month April
     Year of Publication 2020 
     Page 1-15 
     Abstract The Kleeb Bua Daeng formula (KBD) is a Thai traditional medicine for brain health promotion. On the basis of the activities of its individual components, the KBD could have good potential for the treatment of Alzheimer’s disease (AD). Herein, we investigated the KBD as an AD treatment. The ethanol extracts of KBD and its components, i.e., Nelumbo nucifera (NN), Piper nigrum fruits (BP), and the aerial part of Centella asiatica (CA) exhibited antioxidant activity, as determined by both ABTS and DPPH assays. The Ellman’s assay revealed that the KBD, NN, and BP showed an ability to inhibit acetylcholinesterase. The thioflavin T assay indicated that the KBD, NN, BP, and CA inhibited beta-amyloid aggregation. The neuroprotection and Western blot analysis revealed that the KBD reduced H2O2-induced neuronal cell death by inhibiting the expression of pro-apoptotic factors, i.e., cleaved caspase-9 and -3, p-P65, p-JNK, and p-GSK-3β, as well as by inducing expression of anti-apoptotic factors, i.e., MCl1, BClxl, and survivin. Furthermore, the KBD could improve scopolamine induced memory deficit in mice. Our results illustrate that the KBD with multimode action has the potential to be employed in AD treatment. Thus, the KBD could be used as an alternative novel choice for the prevention and treatment of patients with AD. 
     Keyword antioxidant; acetylcholinesterase inhibition; beta-amyloid aggregation; neuroprotection; Nelumbo nucifera; Piper nigrum; Centella asiatica 
Author
605150043-5 Miss CHANTHA CHHENG [Main Author]
Pharmaceutical Sciences Master's Degree

Reviewing Status มีผู้ประเมินอิสระ 
Status ตีพิมพ์แล้ว 
Level of Publication นานาชาติ 
citation true 
Part of thesis true 
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