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Journal Publication
Title of Article Rice bran hydrolysates induce immunomodulatory effects by suppression of chemotaxis and modulation of cytokine release and cell-mediated cytotoxicity 
Date of Acceptance 15 May 2020 
Journal
     Title of Journal Asian Pacific Journal of Tropical Biomedicine 
     Standard SCOPUS 
     Institute of Journal Editorial Office of Hainan Medical University 
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     Year of Publication 2020 
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     Abstract Objective: To evaluate the immunomodulatory effects of rice bran hydrolysates on cultured immune cells and their underlying mechanism. Methods: Rice bran hydrolysates were prepared from pigmented rice (Oryza sativa L.) by hydrothermolysis and protease digestion. Rice bran hydrolysates were assayed for phenolic content and antioxidant activity. Cell proliferation of Jurkat, THP-1 and peripheral blood mononuclear cells (PBMC) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Chemotaxis was evaluated by Transwell chamber methods. Immunoadherence of THP-1 was performed on cultured human umbilical vein endothelial cells (HUVEC) cells. Cytokine released from PBMC was measured by ELISA assay kits. Lymphocytemediated cytotoxicity was carried out on KKU-425 cells. Proteins associated with immunomodulation were analyzed by Western immunoblotting assay. Results: Rice bran hydrolysates were rich in phenolic compounds, such as ferulic acid, catechin, quercetin, and quercetin glycosides. Rice bran hydrolysates suppressed phytohemagglutinin (PHA)- stimulated proliferation of PBMC and Jurkat cells, chemotaxis of Jurkat and THP-1 cells, and immunoadherence of THP-1 on HUVEC cultured cells. The cellular mechanism of rice bran hydrolysates involved the activation of AMPK as well as suppression of mTOR, NF-κB and VCAM-1. Rice bran hydrolysates potentiated PBMC on the PHA-stimulated release of IL-2, TNF-α, IL-4 cells, and IFN-γ and enhanced PHA-induced non-MHC-restricted cytotoxicity on KKU-425 cancer cells. Conclusions: The immunomodulatory effect of phytochemicals derived from rice bran hydrolysates suggests its therapeutic potential for further investigation. 
     Keyword Rice bran; Immunomodulatory; Non-MHCrestricted cell cytotoxicity; Quercetin glycoside; Ferulic acid;Immunoadherence 
Author
587070017-3 Mrs. SUPHANTHIP PHUSRISOM [Main Author]
Medicine Doctoral Degree

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Level of Publication นานาชาติ 
citation false 
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