|
|
Publication
|
| Title of Article |
Anti-inflammatory activity of the dietary supplement Houttuynia cordata fermentation product in RAW264.7 cells and Wistar rats |
| Date of Acceptance |
4 March 2020 |
| Journal |
| Title of Journal |
PLos ONE |
| Standard |
ISI |
| Institute of Journal |
Public Library of Science |
| ISBN/ISSN |
1932-6203 |
| Volume |
15 |
| Issue |
3 |
| Month |
March |
| Year of Publication |
2020 |
| Page |
1-18 |
| Abstract |
Houttuynia cordata Thunb. has been used as a traditional medicine to treat a variety of ailments in Asian countries such as China, Japan, South Korea, and Thailand. In Thailand,
H. cordata fermentation products (HCFPs) are commercially produced and popularly consumed throughout the country without experimental validation. Anti-inflammatory activity of
H. cordata fresh leaves or aerial parts has previously been reported, however, the antiinflammatory activity of the commercially available HCFPs produced by the industrialized
process has not yet been investigated. The aim of this study was to evaluate in vitro and
in vivo anti-inflammatory potential of the selected industrialized HCFP. LPS-induced
RAW264.7 and carrageenan-induced paw edema models were used to evaluate the antiinflammatory activity of HCFP. The phenolic acid components of HCFP aqueous and
methanolic extracts were investigated using HPLC analysis. In RAW264.7 cells, the HCFP
aqueous and methanolic extracts reduced NO production and suppressed LPS-stimulated
expression of PGE2, iNOS, IL-1β, TNF-α and IL-6 levels in a concentration-dependent manner, however, less effect on COX-2 level was observed. In Wistar rats, 3.08 and 6.16 mL/kg
HCFP reduced paw edema after 2 h carrageenan stimulation, suggesting the second phase
anti-edematous effect similar to diclofenac (150 mg/kg). Whereas, 6.16 mL/kg HCFP also
reduced paw edema after 1 h carrageenan stimulation, suggesting the first phase antiedematous effect. Quantitative HPLC revealed the active phenolic compounds including
syringic, vanillic, p-hydroxybenzoic and ferulic acids, which possess anti-inflammatory activity. Our results demonstrated for the first time the anti-inflammatory activity of the industrialized HCFP both in vitro and in vivo, thus validating its promising anti-inflammation potentia |
| Keyword |
Anti-inflammation; COX-2, Houttuynia cordata Thunb.; iNOS; Nitric oxide; PGE2 |
| Author |
|
| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย