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Publication
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| Title of Article |
LUEM PUA RICE EXTRACT ATTENUATES THE SEVERITY OF DSS-INDUCED ULCERATIVE COLITIS IN RATS THROUGH CHOLINOMIMETIC EFFECTS |
| Date of Acceptance |
13 May 2020 |
| Journal |
| Title of Journal |
International Journal of Applied Pharmaceutics (Int J App Pharm) |
| Standard |
SCOPUS |
| Institute of Journal |
Innovare Academic Sciences Pvt. Ltd. |
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| Year of Publication |
2020 |
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| Abstract |
Objective: To investigate the effect of aqueous extract of unpolished dark purple glutinous Thai rice variety Luem Pua (LP) in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in rat and the possible cholinomimetic effects of the extract. Methods: The effect of LP extract (0.5, 1 or 1.5 mg/ml final concentration) on ileum contraction was tested using isolated guinea pig ileum. Certain agonists (acetylcholine, tetramethylammonium and clonidine) and antagonists (hexamethonium chloride and atropine) were studied to determine the cholinomimetic effect of the extract. The effects of LP extract (5 g/kg/day) in DSS-induced UC model (drinking water was replaced with 3% DSS in water for 7 days) in rat were evaluated. On each day of treatment, the change of disease activity index (DAI) were recorded. At the end of the experiments, rats were terminated, disease severity expressed as disease activity index (DAI), colon length and spleen weight were determined.
Results: LP extract at the concentration of 0.5, 1 and 1.5 mg/ml (final concentration) could contract the ileum in a dose-dependent manner and also could be completely blocked by atropine. Oral administration of LP extract could significantly attenuate the severity of DSS-induced UC as seen by the reduction of DAI, colon length and spleen weight.
Conclusion: Results in isolated guinea pig ileum suggest that LP might contain active substance that could activate muscarinic receptors. In addition to antioxidant activity, through activation of muscarinic receptor, might explain the protective effects of LP extract against DSS-induced UC in rats. |
| Keyword |
Keywords: Luem Pua rice, muscarinic effect, ulcerative colitis (UC), dextran sulfate sodium (DSS), disease activity index (DAI). |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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