2012 ©
Journal Publication
Title of Article Evaluation of 3 (4, 5 dimethyl-2-thiazolyl) -2, 5 diphenyl tetrazolium bromide-based method for rapid detection of vancomycin-resistant enterococci  
Date of Acceptance 14 October 2020 
     Title of Journal ScienceAsia 
     Standard ISI 
     Institute of Journal The Science Society of Thailand under Royal Patronage and the National Research Council of Thailand (NRCT) 
     ISBN/ISSN 1513-1874 
     Month -
     Year of Publication 2020 
     Abstract Vancomycin-resistant enterococci (VRE) have become a global problem of hospital- associated infections. A rapid VRE detection and early isolation of the positive cases is essential for prevention and control of its transmission in hospitals. The aim of this study was to develop and evaluate a simple and rapid method for detection of VRE by using 3 (4, 5 dimethyl-2-thiazolyl) -2, 5 diphenyl tetrazolium bromide (MTT) colorimetric method compared with a standard broth microdilution (BMD) and Etest. A total of 85 Enterococcus species (75 vancomycin-non-susceptible Enterococcus, 2 vancomycin-intermediate and 8 vancomycin-susceptible Enterococcus) were studied. The minimum inhibitory concentrations (MICs) of vancomycin for all isolates were tested by BMD, MTT and Etest methods. The MTT-based method gave results within 9 hrs. with good category agreement, essential agreement, very major, major, and minor errors (100, 92.9, 0, 0 and 7.1% respectively), which were comparable to those of the Etest (97.6, 95.3, 2.4, 0 and 4.7% respectively). The Cohen's kappa coefficient (κ) of the MTT method compared with the BMD was perfect agreement (1.000) (p < 0.001). The MTT method is simple and rapid for early VRE detection to support prevention and control of its spread in hospitals.  
     Keyword - vancomycin resistance, enterococci, Etest, broth microdilution, MTT 
595090005-5 Miss NUCHATAT PANTHASRI [Main Author]
Associated Medical Sciences Master's Degree

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