|
|
Publication
|
| Research Title |
DEVELOPMENT AND EVAUATION OF AN IN-HOUSE COMPETITIVE ELISA FOR
DETECTION OF DEHYDROEPIANDROSTERONE SULFATE |
| Date of Distribution |
6 October 2020 |
| Conference |
| Title of the Conference |
The 46th International Congress on Science, Technology and Technology-based Innovation |
| Organiser |
he Science Society of Thailand under the Patronage of His Majesty the King |
| Conference Place |
มหาวิทยาลัยรามคำแหง |
| Province/State |
กรุงเทพมหานคร |
| Conference Date |
5 October 2020 |
| To |
7 October 2020 |
| Proceeding Paper |
| Volume |
46 |
| Issue |
1 |
| Page |
617-624 |
| Editors/edition/publisher |
|
| Abstract |
Dehydroepiandrosterone sulfate ;DHEASͿ is the most abundant circulating steroid hormones which plays
an important role as the initial substance for steroid hormones synthesis͘ The measurement of the DHEAS level is
used as an ͚ agingͲ biomarker͛ to assess the risk of diseases associating with age͘ In general, the enzymeͲ linked
immunosorbent assay ;ELISAͿ is most widely used for DHEAS measurement because of highly specific, rapid, and
suitable detection͘ But the commercial ELISA kit for DHEAS detection has not been produced in Thailand͘ So,
expenses of using imported commercial ELISA kit were quite high and not suitable in resourceͲlimited laboratories͘
Therefore, this study aimed to develop and standardize a costͲ effective inͲ house competitive ELISA for DHEAS
detection͘ An inͲhouse competitive ELISA format was developed and optimized using a polyclonal antiͲDHEAS as the
antibody coating͘ DHEASͲHRP conjugate and 3,3഻,5,5഻Ͳtetramethylbenzidine ;TMBͿ were used as a competitor and
the enzyme mediatorͬsubstrate system, respectively͘ Under optimal conditions, the standard curve was established
from standard DHEAS concentration range of 100 Ͳ 10000 ngͬmL which a linear regression and the limit of detection
was 97͘947 ngͬmL͘ The result covers normal blood levels of DHEAS since the fetus to the age of 59 years old ;120Ͳ
3210 ngͬmLͿ͘ Furthermore, the inͲhouse competitive ELISA was estimated to be 3 times less per test compared to
the cost of imported commercially available ELISA kit͘ |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย