Abstract |
The Start Codon Targeted (SCoT) primer screening for genetic diversity analysis in the genus
Laggera in Thailand was studied to find the suitable primers that can be used for genetic diversity
analysis in Laggera species and population, including L. alata and L. crispata. Thirty-six SCoT primers were used for screening procedure. The results showed that 12 of 36 SCoT primers produced most
polymorphic and clear bands, including primers SCoT2, SCoT4, SCoT5, SCoT6, SCoT15, SCoT17, SCoT24,
SCoT25, SCoT27, SCoT29, SCoT32 and SCoT35. These primers could be generated the number of band
ranged from 11 (SCoT6) to 22 (SCoT15) bands. The size of band ranged from 150 to 3,000 base pairs.
The polymorphic information content (PIC) ranged from 0.1601 (SCoT4) to 0.4306 (SCoT27). Thus, this
study revealed that these 12 primers are suitable for further study on the genetic diversity of Laggera
species and populations in Thailand and they might be useful for the other plant taxa in the family
Asteraceae. |