บทคัดย่อ |
studies have been conducted on the growth promotion of sunchoke by endophytic fungi. This research was the
first to characterize plant growth promoting properties of endophytic fungi including, Macrophomina phaseolina
BUP2/3 and Diaporthe phaseolorum BUP3/1 isolated from sunchoke and Daldinia eschscholtzii 2NTYL11, Trichoderma
koningii ST-KKU1, Trichoderma erinaceum ST-KKU2, Macrophomina phaseolina SS1L10 and Macrophomina
phaseolina SS1R10 from medicinal plants. Also, their plant growth promoting efficiency in artichoke plants under
greenhouse condition was evaluated. The highest phosphate solubility and production of extracellular enzymes
including amylase, protease, cellulase and xylanase were found after 5 and 21 days of incubation, respectively.
Moreover, Indole-3-Acetic Acid (IAA) in the crude extracts of fungi isolated from Jerusalem artichoke was higher
than that from medicinal plants (p ≤ 0.05). HPLC analysis suggested the putative IAA biosynthesis pathways in
our fungi were via Indole-3-lactic acid (ILA) and Indole-3-acetamide (IAM). In the pot experiment, the values of
height, diameter, chlorophyll content and leaf dry weight in sunchoke plants inoculated with fungal endophytes
were significantly higher than those of un-inoculated plants. Therefore, this study suggested that fungal endophytes
could be used as a biofertilizer for promoting growth of sunchoke. |