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Publication
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| Title of Article |
A chemical marker determination in Dipterocarpus alatus oleoresin samples and bioactivities screening via antioxidants, nitric oxide inhibition on murine Raw 264.7 cells, and collagen production on normal human dermal fibroblasts. |
| Date of Acceptance |
30 March 2021 |
| Journal |
| Title of Journal |
the Tropical Journal of Natural Product Research |
| Standard |
SCOPUS |
| Institute of Journal |
Natural Product Research Group, University of Benin |
| ISBN/ISSN |
2616-0684 |
| Volume |
5 |
| Issue |
4 |
| Month |
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| Year of Publication |
2021 |
| Page |
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| Abstract |
It has been shown that (-)-alpha-gurjunene was the major component in oleoresin of
Dipterocarpus alatus. In this study, (-)-alpha-gurjunene was investigated from 5 samples of
D. alatus oleoresin which were prepared from different methods and different sources by a
validated HPLC-UV technique. Furthermore, the biological activities such as, antioxidation,
nitric oxide inhibition on murine Raw 264.7 cells, and collagen production activity on normal
human dermal fibroblasts (NHDFs) were also investigated. Our study found that (-)-alphagurjunene content from D. alatus oleoresin samples were obtained was from 7.32 to 17.35
mg/g sample. Low antioxidant activities of D. alatus oleoresins were found when evaluated
by ABTS, DPPH and FRAP assay, however, D. alatus oleoresins could inhibit nitric oxide
(NO) production on Raw 264.7 cells. The 2.5 µg/mL of supernatant after degumming oleoresin
showed the highest NO inhibition (71.89%). In addition, the heated oleoresin could stimulate
higher collagen production on NHDFs than ascorbic acid at levels 3 times higher than the
negative control. Therefore, different oleoresin preparation methods affected the chemical
contents and biological activities such as antioxidation, nitric oxide inhibition etc. Our results
showed that D. alatus oleoresin has potential to be developed as an anti-inflammatory or
wound healing product(s), however, its efficacy requires investigation |
| Keyword |
Dipterocarpus alatus oleoresin, (-)-alpha-gurjunene, anti-inflammatory, collagen production activity |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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