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Publication
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| Title of Article |
AuNPs-LISA, an efficient detection assay for Opisthorchis viverrini (Ov) antigen in urine |
| Date of Acceptance |
24 November 2019 |
| Journal |
| Title of Journal |
Talanta |
| Standard |
SCOPUS |
| Institute of Journal |
Elsevier BV |
| ISBN/ISSN |
0039-9140 |
| Volume |
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| Issue |
209 |
| Month |
Mar |
| Year of Publication |
2020 |
| Page |
120592 |
| Abstract |
The enzyme-linked immunosorbent assay (ELISA) is currently a powerful technique for the detection of
Opisthorchis viverrini antigen (OvAg) in urine samples. However, its sensitivity and analysis time need to be
improved. In the present study, we aimed to improve the signal enhancing system of traditional ELISA by using gold nanoparticles (AuNPs) with peroxidase-like activity on its surface instead of the horseradish peroxidase (HRP) system. The catalytic activity of the AuNPs probe can be boosted by the gold enhancing solution and the addition of ATP. The catalytic ability of the AuNPs probe depended on the probe and the H2O2 concentration.The proposed approach can reduce the number of the traditional ELISA steps with better detection sensitivity. Interestingly, the limit of detection (LOD) of the test was 23.4 ng mL−1, substantially lower than the
93.8 ng mL−1 for the traditional ELISA. The AuNPs-LISA assay showed higher sensitivity and specificity, 93.81% and 91.34%, respectively, compared to the traditional ELISA. The proposed assay was successfully applied for the detection of OvAg in urine samples. This will provide an effective tool for the detection, control and elimination of human opisthorchiasis. |
| Keyword |
Opisthorchiasis; Gold nanoparticles (AuNPs); Cholangiocarcinoma (CCA); Peroxidase-like activity; Nano ELISA |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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