2012 ©
Journal Publication
Title of Article A fluorescence AuNPs-LISA: A new approach for Opisthorchis viverrini (Ov) antigen detection with a simple fluorescent enhancement strategy by surfactant micelle in urine samples 
Date of Acceptance 15 February 2021 
     Title of Journal Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 
     Standard SCOPUS 
     Institute of Journal Elsevier 
     ISBN/ISSN 13861425 
     Issue 254 
     Month June
     Year of Publication 2021 
     Page 119633 
     Abstract The colorimetric AuNPs-LISA is a new, powerful technique for the detection of Opisthorchis viverrini antigen (OvAg) in urine samples. However, the diagnostic sensitivity of the colorimetric AuNPs-LISA is powerless to screen ultralow concentrations of OvAg in urine samples in cases of early stage liver fluke infection. This work, we aimed to improve the diagnostic sensitivity of the colorimetric AuNPs-LISA by developing a new fluorescence AuNPs-LISA. O-phenylenediamine (OPD) was used as the chromogenic substrate instead of the tetramethylbenzidine (TMB) of the colorimetric AuNPs-LISA. Interestingly, the fluorescence of the OPD oxidation product by the peroxidase-like activity of labelled AuNPs can be extremely enhanced by a non-ionic surfactant, especially the Triton X-100. The proposed assay exhibited a dynamic linear detection of OvAg concentration in the range of 34.18 ng mL-1 to 273.44 ng mL-1 with the limit of detection at 36.97 ng mL-1 which the detection sensitivity enhancement around 1200-fold when comparing with the colorimetric AuNPs-LISA. This model exhibits high diagnosis sensitivity. 
     Keyword Surfactant; Fluorescent enhancement; Opisthorchiasis; Cholangiocarcinoma (CCA); Peroxidase-like activity 
617070019-2 Mr. WICHIT TARON [Main Author]
Medicine Doctoral Degree
Medicine Doctoral Degree

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Level of Publication นานาชาติ 
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