2012 ©
Journal Publication
Title of Article Diagnostic and Prognostic Value of Circulating Cell‐Free DNA for Cholangiocarcinoma 
Date of Acceptance 28 May 2021 
     Title of Journal Diagnostics 
     Standard ISI 
     Institute of Journal Multidisciplinary Digital Publishing Institute (MDPI) 
     Volume 2021 
     Issue 11 
     Month May
     Year of Publication 2021 
     Abstract The analysis of cfDNA has been applied as a liquid biopsy in several malignancies. However, its value in the diagnosis and prognosis of cholangiocarcinoma (CCA) have not been well defined. We aimed to investigate the diagnostic and prognostic values of cfDNA level and tumor-specific mutation in circulating DNA (ctDNA) in CCA. The plasma cfDNA levels from 62 CCA patients, 33 benign biliary disease (BBD) patients and 30 normal controls were quantified by fluorescent assay. Targeted probe-based sequencing of 60 genes was applied for mutation profiling in 10 ctDNA samples and their corresponding treatment-naïve tissues. cfDNA levels in CCA were significantly higher than those in BBD and normal controls. We found that cfDNA levels at 0.2175 and 0.3388 ng/µL significantly discriminated CCA from healthy controls and BBD with 88.7 and 82.3% sensitivity and 96.7 and 57.6% specificity, respectively. cfDNA levels showed superior diagnostic efficacy in detecting CCA compared to CEA and CA19-9. ARID1A (30%), PBRM1 (30%), MTOR (30%), and FGFR3 (30%) mutations were the most common. Using nine frequently mutated genes in the ctDNA samples, the diagnostic accuracy of cfDNA sequencing was 90.8%, with 96.7% average sensitivity and 72.4% specificity. This study supports the use of cfDNA as a diagnosis and prognostic biomarker for CCA. 
     Keyword bile duct cancer; cell-free DNA; circulating tumor DNA; prognosis; diagnosis; liquid biopsy; sequencing 
615100003-8 Miss PREAWWALEE WINTACHAI [Main Author]
Graduate School Master's Degree

Reviewing Status มีผู้ประเมินอิสระ 
Status ตีพิมพ์แล้ว 
Level of Publication นานาชาติ 
citation false 
Part of thesis true 
Attach file
Citation 0