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Publication
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| Research Title |
Serum Cytokine-induced apoptosis inhibitor 1 level as a diagnostic biomarker for cholangiocarcinoma |
| Date of Distribution |
18 December 2020 |
| Conference |
| Title of the Conference |
The 1st CARI Annual Conference 2020 |
| Organiser |
Cholangiocarcinoma research institute, Faculty of Medicine, KKU |
| Conference Place |
LE CASSIA HOTEL |
| Province/State |
Khon Kaen, Thailand |
| Conference Date |
18 December 2020 |
| To |
19 December 2020 |
| Proceeding Paper |
| Volume |
None |
| Issue |
None |
| Page |
None |
| Editors/edition/publisher |
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| Abstract |
Introduction: The mortality rate of cholangiocarcinoma (CCA) is high since there is a lack of non-invasive approaches able to accurately detect this silent tumor at early stages, and its diagnostic and/or prognostic biomarkers are still insufficient with poor prognosis of patients. To discover new biomarkers, we re-analyzed our mitochondrial proteome database of CCA tissues by using bioinformatics tools for secretory protein nature. Cytokine-induced apoptosis inhibitor 1 (CIAPIN1), was predicted that it was lacking signal peptide and might be secreted into plasma/serum via an unconventional pathway, was selected for the present study.
Methods: Checking expression of CIAPIN1 in tissue and serum of intrahepatic Cholangiocarcinoma (iCCA) patients were analyzed by western blot. CIAPIN1 levels in the pre-operative sera of 46 iCCA patients and 23 healthy controls (HC) were measured using a semi-quantitative dot blot assay. Diagnostic values of serum CIAPIN1 levels were evaluated.
Results: The median level ± quartile deviation of CIAPIN1 was 0.31 ± 0.22 µg/µL and 0.66 ± 0.30 µg/µL in the HC group and iCCA patient group, respectively. CIAPIN1 levels in the sera of iCCA patients were significantly higher than those of healthy controls (p < 0.0001, Mann–Whitney U test). Using a cut-off value of 0.46 µg/µL (at maximal Youden Index), CIAPIN1 provided 71.74% sensitivity, 95.65 % specificity, 79.71 accuracy, and the area under the curve (AUC) was 0.863 (95%CI: 0.779-0.947) for diagnosis of iCCA.
Conclusion: CIAPIN1 might be used as a diagnostic marker for CCA. Furthermore, the expression level of CIAPIN1 in the serum of benign biliary disease patients compare with those of pre-operative serum samples of iCCA patients need to be investigated in the future.
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
true |
| Award Title |
The best poster presentation |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
CARI, Faculty of Medicine, KKU |
| Date of awarding |
19 ธันวาคม 2563 |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย