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Publication
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| Title of Article |
Fragment antigen‑binding (Fab) antibody‑based lateral flow
immunoassay for rapid and sensitive detection of potent
phytoestrogen, deoxymiroestrol |
| Date of Acceptance |
2 June 2021 |
| Journal |
| Title of Journal |
Journal of Natural Medicines |
| Standard |
SCOPUS |
| Institute of Journal |
Springer |
| ISBN/ISSN |
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| Volume |
Article in press |
| Issue |
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| Month |
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| Year of Publication |
2021 |
| Page |
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| Abstract |
Pueraria candollei is an ingredient of Thai herbal medicine, dietary supplements, and cosmetics. The in vitro and in vivo
studies of this plant supported anti-osteoporotic activity and used for hormone replacement therapy. Deoxymiroestrol shows
the most potent phytoconstituent in tuberous root of P. candollei with estrogenic activity. The quality controls are important
for good agricultural practice (GAP) and good manufacturing practice (GMP) of plant-derived raw materials. The rapid
detection of lateral flow immunoassay (LFIA) using colloidal gold is simply method, easy visualize detection and produce
less waste than conventional chromatographic detection. In this study, LFIA for qualitative detection of deoxymiroestrol using
antigen-binding fragment antibody (Fab) was developed. The result showed that the developed LFIA displays specific detection
of deoxymiroestrol. Cross reactivity of this method was analyzed with miroestrol, isomiroestrol and methylisomiroestrol
which showed 39.97%, 7.71% and 5.72%, respectively. After optimal condition, limit of detection (LOD) for deoxymiroestrol
is 250 ng/ml. Plant samples were applied to strip test compare with indirect competitive ELISA using polyclonal antibody
to confirm the application of LFIA. The results of LFIA method were comparable with those from ELISA. This developed
lateral flow immunoassay can apply to detect deoxymiroestrol for the rapid testing. The developed method can use for quality
control in plant samples as deoxymiroestrol is biomarker compound in P. candollei. |
| Keyword |
Deoxymiroestrol, Fragment antigen-binding (Fab) antibody, Lateral flow immunoassay (LFIA), Pueraria candollei |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย