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Publication
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| Title of Article |
Effects of Hydrolysable Tannins Extract from Sweet Chestnut Wood (Castanea Sativa Mill.) on Subclinical Mastitis Pathogens from Thai Friesian Dairy Cows |
| Date of Acceptance |
4 August 2021 |
| Journal |
| Title of Journal |
Veterinary World |
| Standard |
SCOPUS |
| Institute of Journal |
Veterinary World |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Year of Publication |
2021 |
| Page |
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| Abstract |
Background and Aim: This study was to investigate hydrolysable tannins from sweet chestnut wood on bacterial pathogens from infecting Thai Friesian dairy cows with subclinical mastitis. The objective of this investigation was to assess how efficient a hydrolysable tannin extract obtained from sweet chestnut wood (Castanea sativa Mill.) was at inhibiting mastitis-causing bacteria in vitro.
Materials and Methods: The negative control was sterile water, whereas the positive controls were penicillin and gentamicin, with hydrolysable tannins as the assigned treatments. The treatments included five doses of hydrolysable tannins at 63, 190, 313, 630, and 940 mg/mL, respectively. The bacteria causing mastitis were isolated and identified in subclinical mastitis cows. The antibacterial activity of the hydrolysable tannins was assessed by the disk diffusion method, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay were also measured.
Results: The results showed that penicillin inhibited (P<0.01) S. aureus, S. uberis and P. aeruginosa growth but could not inhibit (P>0.05) S. agalactiae, E. coli and K. pneumonia growth, while gentamicin and hydrolysable tannins could inhibit (P<0.01) all isolated bacteria. When hydrolysable tannins doses increased resulted in quadratic increased the inhibition zone diameters of S. aureus and S. agalactiae while linearly increased that of E. coli, K. pneumonia, and P. aeruginosa. The hydrolysable tannins at 630 and 940 mg/mL showed the highest antibacterial activity for S. agalactiae and E. coli (P<0.01), while the hydrolysable tannins at 940 mg/mL had the highest antibacterial activity for K. pneumonia (P<0.01). The MIC and MBC of the hydrolysable tannins were 37.5-300 mg/mL, and 93.8-375.0 mg/mL, respectively, with the MBC: MIC ratio was 2:1.
Conclusion: Antimicrobial activity of hydrolysable tannins against subclinical mastitis bacteria was comparable to antibiotics at concentrations over 630 mg/mL. The in vitro findings are promising, but more research is needed to determine whether hydrolysable tannins could be applied to control or prevent subclinical mastitis in dairy cows.
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| Keyword |
hydrolysable tannins, subclinical mastitis, pathogen, Thai Friesian dairy cows |
| Author |
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| Reviewing Status |
ไม่มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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