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Publication
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| Title of Article |
Neuroprotective Assessment of Moringa oleifera Leaves Extract against Oxidative‐Stress‐Induced Cytotoxicity in SHSY5Y Neuroblastoma Cells |
| Date of Acceptance |
26 April 2021 |
| Journal |
| Title of Journal |
Plants |
| Standard |
SCOPUS |
| Institute of Journal |
MDPI (Basel, Switzerland) |
| ISBN/ISSN |
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| Volume |
10 |
| Issue |
5 |
| Month |
April |
| Year of Publication |
2021 |
| Page |
889 |
| Abstract |
The current trend worldwide is searching plant extracts towards prevention of
neurodegenerative disorders. This study aimed to investigate the neuroprotective effect of Alpinia
galanga leaves (ALE), Alpinia galanga rhizomes (ARE), Vitis vinifera seeds (VSE), Moringa oleifera
leaves (MLE), Panax ginseng leaves (PLE) and Panax ginseng rhizomes (PRE) ethanolic extracts on
human neuroblastoma (SHSY5Y) cells. The 1‐diphenyl‐1‐picrylhydrazyl (DPPH) radical
scavenging of VSE and MLE were 81% and 58%, respectively. Ferric‐reducing antioxidant power
(FRAP) of ALE and MLE (33.57 ± 0.20 and 26.76 ± 0.30 μmol Fe(ΙΙ)/g dry wt., respectively) were
higher than for the other extracts. Liquid chromatography coupled to quadrupole time‐of‐flight
mass spectrometry (LC‐QTOF/MS) revealed MLE active compounds. Intracellular study by nitroblue
tetrazolium (NBT) test showed that MLE and VSE had high O2− scavenging (0.83 ± 0.09 vs.
0.98 ± 0.08 mg/mL, respectively). MLE had the highest ROS scavenging followed by PRE (0.71 ±
0.08 vs. 0.83 ± 0.08 mg/mL, respectively), by 2,7‐dichlorodihydrofluorescein diacetate (DCFHDA)
assay. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) cytotoxicity and
neuroprotection tests on SHSY5Y showed that PRE had a better neuroprotective effect but higher
cytotoxicity compared to MLE (viable cells 51% vs. 44%, IC50 1.92 ± 0.04 vs. 2.7 ± 0.2 mg/mL,
respectively). In conclusion, among the studied plants, MLE has potential for developing as a
neuroprotective agent. |
| Keyword |
Moringa oleifera; ROS; H2O2; oxidative stress; superoxide anion; SHSY5Y neuroblastoma cell line |
| Author |
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| Reviewing Status |
ไม่มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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