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Publication
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| Title of Article |
Isolation of Bacteria Utilizing Glycerol as a Sole Carbon Source for Potential Production of 1,3-Propanediol |
| Date of Acceptance |
9 August 2021 |
| Journal |
| Title of Journal |
Asia-Pacific Journal of Science and Technology (APST) |
| Standard |
SCOPUS |
| Institute of Journal |
Research and Technology Transfer Affairs Division of Khon Kaen University |
| ISBN/ISSN |
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| Volume |
27 |
| Issue |
02 |
| Month |
March-April |
| Year of Publication |
2022 |
| Page |
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| Abstract |
Glycerol is a by-product waste from biodiesel production, which can be converted by bacteria to other value-added products. One of these products is 1,3-propanediol, an important substrate for synthesis of biopolymer and many others. This research aims to isolate bacteria that can utilize glycerol as a sole carbon source and examine the 1,3-propanediol production ability of these isolates using glycerol as a substrate. Bacterial isolates were obtained from soil near biodiesel plant by culturing in mineral salt medium containing glycerol. Growth of selected isolates in a medium containing glycerol as a sole carbon source were monitored and compared with reference bacterial strains. It was observed that 2 isolates grew better than reference strains according to optical density value at 600 nm. Both isolates were identified as Klebsiella pneumoniae by 16S rRNA gene sequencing. The isolate 5-3, renamed as K. pneumoniae KKU5 (Kp KKU5) was used for further study. The dhaT gene of Kp KKU5 shared 99% identity with those of K. pneumoniae (CP025630.1). To examine the ability of Kp KKU5 as a 1,3-propanediol producer, its fermentation broth containing glycerol was analyzed by HPLC. Under batch fermentation conditions with 20 g/L initial glycerol, Kp KKU5 produced 0.375 g/L of 1,3-propanediol after 7 days. Despite its low production of 1,3-propanediol, we have demonstrated that Kp KKU5 was able to utilize glycerol as a sole carbon source. This study might provide some useful information to contribute in the research area of 1,3-propanediol production, that adds value to glycerol waste from biodiesel production. |
| Keyword |
1,3-Propanediol, Glycerol utilizing bacteria, Klebsiella pneumoniae, 1,3-propanediol oxidoreductase |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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