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Publication
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| Title of Article |
Localization of phosphatidylinositol 4-phosphate 5-kinase (PIP5K) α confined to the surface of lipid droplets and adjacent narrow cytoplasm in progesterone-producing cells of in situ ovaries of adult mice |
| Date of Acceptance |
29 September 2021 |
| Journal |
| Title of Journal |
Acta Histochemica |
| Standard |
ISI |
| Institute of Journal |
Elsevier GmbH. |
| ISBN/ISSN |
ISSN 0065-1281 |
| Volume |
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| Issue |
ุุ6 |
| Month |
September |
| Year of Publication |
2021 |
| Page |
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| Abstract |
Phosphatidylinositol(4,5)bisphosphate (PI(4,5)P2) produced by phosphatidylinositol
phosphate 5 kinase (PIP5K) plays not only as a precursor of second messengers in the
phosphoinositide signal transduction, but also multiple roles influencing a variety of
cellular activities. From this viewpoint, the present study attempted to localize PIP5Kα in
the ovaries in situ of adult mice. PIP5Kα-immunoreactivity was confined to the surfaces of
lipid droplets (LDs) and their adjacent cytoplasm in progesterone-producing cells of the
interstitial glands, corpora lutea and theca interna. The LDs often contained membranous
tubules/lamellae along their surfaces and within their interior whose membranes were
continuous with those delineating LDs composed of a monolayer of phospholipids and
were partially PIP5Kα-immunoreactive. Although granulosa cells of healthy-looking
follicles were immunonegative, as the atresia progressed, PIP5Kα-immunoreactivity firstappeared in sparsely dispersed dot forms in mural cells of the follicular epithelia, and then
were dominant in almost all mural cells that remained after desquamation of the antral cells.
The present study provides evidence suggesting that PI(4,5)P2 locally synthesized by
PIP5K in LDs is involved in the lipid transfer between lipid droplets (LDs) and the
endoplasmic reticulum, which eventually regulates ovarian progesterone production
through control of multiple dynamic activities of LDs. It is also suggested that PIP5Kα and
PI(4,5)P2 are implicated in the modulation of programmed cell death and/or acquiring the
ability of progesterone production in some follicular cells surviving atresia.
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| Keyword |
PIP5Kα, Lipid droplet, Progesterone-cells, Ovary, Mice |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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